Human FAS knockout HeLa cell line
- Advanced Validation
- What is this?
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FAS KO cell line available to order. KO validated by Western blot. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2.
View Alternative Names
ALPS 1A, ALPS2B, APO 1, APO 1 cell surface antigen, APT-1, Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 12 protein, Apo-1 antigen, Apoptosis antigen 1, Apoptosis regulator BAX, Apoptosis related cysteine peptidase, Apoptosis-mediating surface antigen FAS, Apoptotic cysteine protease, Apoptotic protease Mch-5, BAXA, BAX_HUMAN, BCL2 associated X protein, BCL2 associated X protein omega, BCL2 associated X protein transcript variant delta2, BING 2, Bax-protein, Baxdelta2G9, Baxdelta2G9omega, Baxdelta2omega, Bcl-2-like protein 4, Bcl2-L-4, CAP 4, CARD 3, CARD carrying kinase, CARD containing ICE associated kinase, CARD containing interleukin 1 beta converting enzyme (ICE) associated kinase, CARD-containing IL-1 beta ICE-kinase, CARD-containing interleukin-1 beta-converting enzyme-associated kinase, CARDIAK, CASP8_HUMAN, CD 95, CD 95 antigen, CED 3, CENP-C binding protein, CLARP kinase, Caspase 8, Caspase 8 apoptosis related cysteine peptidase, Caspase IIX, Caspase-8 subunit p10, DAP 6, DAXX_HUMAN, Death associated protein 6, Death domain associated protein, Death domain-associated protein 6, Delta Fas, Delta Fas/APO 1/CD95, EAP 1, ETS1-associated protein 1, FADD protein, FADD-homologous ICE/CED-3-like protease, FADD-like ICE, FADD_HUMAN, FAS 1, FAS 827dupA, FAS Antigen, FAS-associated death domain protein, FAS-associating death domain-containing protein, FASLG receptor, FASTM, FLICE, FLJ17672, Fas (TNF receptor superfamily, member 6), Fas (TNFRSF6) associated via death domain, Fas AMA, Fas TNFRSF6 associated via death domain, Fas associated via death domain, Fas associating protein, Fas associating protein with death domain, Fas binding protein, Fas cell surface death receptor, Fas death domain-associated protein, GIG 3, GIG 30, Growth inhibiting gene 30, Growth-inhibiting gene 3 protein, H sapiens mRNA for mediator of receptor induced toxicity, ICE-like apoptotic protease 5, MACH, MACH alpha 1/2/3 protein, MACH beta 1/2/3/4 protein, MACH5, MCH 5, MGC126245, MGC126246, MGC78473, MGC8528, MORT 1, MORT1-associated CED-3 homolog, Mediator of receptor induced toxicity, OTTHUMP00000163717, OTTHUMP00000163720, OTTHUMP00000163724, OTTHUMP00000163725, OTTHUMP00000165062, OTTHUMP00000165063, OTTHUMP00000165064, OTTHUMP00000206552, OTTHUMP00000206582, Protein FADD, RICK, RIP-like-interacting CLARP kinase, RIPK2_HUMAN, Receptor interacting protein (RIP) like interacting caspase like apoptosis regulatory protein (CLARP) kinase, Receptor interacting serine threonine kinase 2, Receptor-interacting protein 2, Receptor-interacting serine/threonine-protein kinase 2, Surface antigen APO1, TNF receptor superfamily, member 6, TNFRSF, TNFRSF 6, TNR6_HUMAN, Tumor necrosis factor receptor superfamily member 6, Tyrosine-protein kinase RIPK2, UNQ277/PRO314/PRO34092, hDaxx, membrane isoform alpha, sFAS
- WB
Lab
Western blot - Human FAS knockout HeLa cell line (AB265260)
Lanes 1-2 : Merged signal (red and green). Green - ab133619 observed at 37 kDa. Red - loading control ab8245 observed at 37 kDa.
ab133619 Anti-Fas antibody [EPR5700] was shown to specifically react with Fas in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265260(knockout cell lysate ab256911) was used. Wild-type and Fas knockout samples were subjected to SDS-PAGE. ab133619 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-Fas antibody [EPR5700] (<a href='/en-us/products/primary-antibodies/fas-antibody-epr5700-ab133619'>ab133619</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human FAS knockout HeLa cell line (ab265260)
Lane 2:
FAS knockout HeLa cell lysate at 20 µg
Predicted band size: 37 kDa
Observed band size: 37 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human FAS knockout HeLa cell line (AB265260)
Homozygous : 1 bp insertion in exon 2.
- Cell Culture
Unknown
Cell Culture - Human FAS knockout HeLa cell line (AB265260)
Representative images of FAS knockout HeLa cells, low and high confluency examples (top left and right respectively) and wild-type HeLa cells, low and high confluency (bottom left and right respectively) showing typical adherent, epithelial-like morphology. Images were captured at 10X magnification using a EVOS XL Core microscope.
Reactivity data
Product details
Recommended control: Human wild-type HeLa cell line (ab255928). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Target data
Complementary Products
Primary Antibodies
AB271016
Anti-Fas antibody [EPR24898-74]
BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|Trial Size|Trial Size
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fas antibody [EPR24898-74] (AB271016)](https://content.abcam.com/products/images/fas-antibody-epr24898-74-ab271016--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img172013.jpg)
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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