Human FCGR1A knockout THP-1 cell line
Be the first to review this product! Submit a review
|
(0 Publication)
- WB
Lab
Western blot - Human FCGR1A knockout THP-1 cell line (AB275843)
False colour image of Western blot : Anti-CD64 antibody [EPR4623] staining at 1/1000 dilution shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution shown in red. In Western blot ab109449 was shown to bind specifically to CD64. A band was observed at 45-50/55-80 kDa in wild-type THP-1 cell lysates with no signal observed at this size in FCGR1A knockout cell line ab275843 (knockout cell lysate ab275817). To generate this image wild-type and FCGR1A knockout THP-1 cell lysates were analysed. First samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °. Blots were washed four times in TBS-T incubated with secondary antibodies for 1 h at room temperature washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
All lanes:
Western blot - Anti-CD64 antibody [EPR4623] (<a href='/en-us/products/primary-antibodies/cd64-antibody-epr4623-ab109449'>ab109449</a>) at 1/1000 dilution
Lane 1:
Wild-type THP-1 cell lysate at 20 µg
Lane 2:
FCGR1A knockout THP-1 cell lysate at 20 µg
Lane 2:
Western blot - Human FCGR1A knockout THP-1 cell line (ab275843)
Lane 3:
U937 cell lysate at 20 µg
Lane 4:
HEK-293 cell lysate at 20 µg
Predicted band size: 43 kDa
Observed band size: 45-50 kDa
false
- WB
Lab
Western blot - Human FCGR1A knockout THP-1 cell line (AB275843)
False colour image of Western blot : Anti-CD64 antibody [EPR4624] staining at 1/10000 dilution shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution shown in red. In Western blot ab134073 was shown to bind specifically to CD64. A band was observed at 45-50/55-80 kDa in wild-type THP-1 cell lysates with no signal observed at this size in FCGR1A knockout cell line ab275843 (knockout cell lysate ab275817). To generate this image wild-type and FCGR1A knockout THP-1 cell lysates were analysed. First samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °. Blots were washed four times in TBS-T incubated with secondary antibodies for 1 h at room temperature washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
All lanes:
Western blot - Anti-CD64 antibody [EPR4624] (<a href='/en-us/products/primary-antibodies/cd64-antibody-epr4624-ab134073'>ab134073</a>) at 1/10000 dilution
Lane 1:
Wild-type THP-1 cell lysate at 20 µg
Lane 2:
FCGR1A knockout THP-1 cell lysate at 20 µg
Lane 2:
Western blot - Human FCGR1A knockout THP-1 cell line (ab275843)
Lane 3:
U937 cell lysate at 20 µg
Lane 4:
HEK-293 cell lysate at 20 µg
Predicted band size: 43 kDa
Observed band size: 45 kDa,45-50 kDa
false
- NGS
Supplier Data
Next Generation Sequencing - Human FCGR1A knockout THP-1 cell line (AB275843)
Knockout achieved by CRISPR/Cas9; X = 1 bp deletion, 2 bp deletion; Frameshift : 99.99%
- NGS
Supplier Data
Next Generation Sequencing - Human FCGR1A knockout THP-1 cell line (AB275843)
1 bp (allele 1) or 2 bp deletion (allele 2) after Asn 77 of the WT protein
Complementary Products
![Immunoprecipitation - Anti-CD64 antibody [EPR4624] (AB134073)](https://content.abcam.com/products/images/cd64-antibody-epr4624-ab134073--immunoprecipitation-img173278.jpg)
Reactivity data
Product details
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method. Based on cell count, seed cells in an appropriate cell culture flask at a density of 4x105 cells/mL. Seeding density is given as a guide only and should be scaled to align with individual lab schedules.
4. Incubate the culture at 37°C incubator with 5% CO2. Cultures should be monitored daily.
5. THP-1 cells recover slowly from cryopreservation and therefore may not be ready for subculture for a number of days. Cells should be left as much as possible over this time and only subcultured when the cell density reaches 8x105 cells/mL.Small amounts of fresh media can be added until cell number/viability improves.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- Cells should be seeded at 2x105 - 3x105 cells/mL and subcultured when they have reached 8x105 cells/mL.
- It is not recommended to allow the cell density to exceed 1x106 cells/mL.
Culture medium
RPMI + 10% FBS + 0.05 mM beta-mercaptoethanol
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
CD64 expression involves the binding of IgG which serves as a pivotal mechanism to facilitate phagocytosis and clearance of opsonized pathogens. CD64 does not operate solely; it is part of a larger receptor family that includes other Fc gamma receptors like CD16 and CD32. The regulation of CD64 differs from its relatives due to its higher affinity for IgG. This attribute enables CD64 to play a more significant role in triggering immune responses especially during infections and inflammatory processes.
Pathways
CD64 participates in the immune response pathway and is critical in the regulation of inflammatory responses. Key proteins interacting with CD64 in these pathways include the aforementioned CD16 and CD32 forming a network that ensures efficient immune system activation. The interaction with IgG and subsequent signal transduction underpin the receptor's functionality in these pathways harmonizing innate and adaptive immune responses.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Suspension
Gender
Male
Target data
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com