Human FGFR3 knockout HEK-293 cell line
- Advanced Validation
- What is this?
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FGFR3 KO cell line available to order. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control available. Knockout achieved by CRISPR/Cas9 X = 1 bp insertion Frameshift = 99.28%. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
View Alternative Names
ACH, CD 333, CD333 antigen, CEK 2, FGFR3_HUMAN, Fibroblast growth factor receptor 3, Fibroblast growth factor receptor 3 (achondroplasia thanatophoric dwarfism), HSFGFR3EX, JTK 4, MFR 3, SAM 3, Tyrosine kinase JTK 4, Z FGFR 3, heparin-binding growth factor receptor, hydroxyaryl-protein kinase
- WB
Lab
Western blot - Human FGFR3 knockout HEK-293 cell line (AB259775)
Lanes 1 - 3 : Merged signal (red and green). Green - ab133644 observed at 125kDa. Red - loading control ab9484 observed at 37 kDa.
ab133644 was shown to recognize in wild-type HEK-293 cells as signal was lost at the expected MW in FGFR3 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and FGFR3 knockout samples were subjected to SDS-PAGE. ab133644 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-FGFR3 antibody [EPR2304(3)] (<a href='/en-us/products/primary-antibodies/fgfr3-antibody-epr23043-ab133644'>ab133644</a>) at 1/1000 dilution
Lane 1:
Wild-type HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg
Lane 2:
FGFR3 knockout HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg
Lane 2:
Western blot - Human FGFR3 knockout HEK-293 cell line (ab259775)
Lane 3:
Hep G2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg
Predicted band size: 76 kDa,88 kDa
Observed band size: 125 kDa
false
- NGS
Supplier Data
Next Generation Sequencing - Human FGFR3 knockout HEK-293 cell line (AB259775)
1 bp deletion after Gly51 of the WT protein
- NGS
Supplier Data
Next Generation Sequencing - Human FGFR3 knockout HEK-293 cell line (AB259775)
Knockout achieved by CRISPR/Cas9; X = 1 bp insertion; Frameshift = 99.28%
Reactivity data
Product details
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
FGFR3 participates in important processes such as chondrogenesis and osteogenesis which are essential for bone growth and development. The receptor operates within a complex system involving interactions with ligand-induced dimerization and autophosphorylation. This phosphorylation event activates the receptor's intrinsic kinase activity further triggering downstream signaling pathways for cellular responses.
Pathways
FGFR3 is involved with the MAPK/ERK and PI3K/AKT pathways which play significant roles in cell proliferation differentiation and survival. FGFR3 links closely with FGF ligands and similar receptor types such as FGFR1 and FGFR2 within these pathways. Differences in pathway activation can lead to diverse biological responses illustrating its pivotal role in maintaining cellular homeostasis.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Target data
Complementary Products
Primary Antibodies
AB133644
Anti-FGFR3 antibody [EPR2304(3)]
RabMAb|Recombinant|KO Validated|20ul selling size
![Western blot - Anti-FGFR3 antibody [EPR2304(3)] (AB133644)](https://content.abcam.com/products/images/fgfr3-antibody-epr23043-ab133644--western-blot-img47321.jpg)
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com