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FHL1 KO cell line available to order. KO validated by Western blot. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 4 and Insertion of the selection cassette in exon 4.

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Images

Western blot - Human FHL1 knockout HeLa cell line (AB266011), expandable thumbnail
  • Sanger Sequencing - Human FHL1 knockout HeLa cell line (AB266011), expandable thumbnail
  • Sanger Sequencing - Human FHL1 knockout HeLa cell line (AB266011), expandable thumbnail

Key facts

Cell type
HeLa
Species or organism
Human
Tissue
Cervix
Form
Liquid
Knockout validation
Sanger Sequencing, Western blot
Mutation description
Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 4 and Insertion of the selection cassette in exon 4

Alternative names

FHL 1B, FHL1 protein, FHL1A, FHL1_HUMAN, FLH1A, Four and a half LIM domains 1, Four and a half LIM domains protein 1, Four and a half Lin11 Isl 1 and Mec 3 domains 1, Isl-1 and Mec-3, KYO T, KYOT, mouse, homolog of, LIM protein SLIMMER, MGC111107, RAM14-1, RBMX1A, RBMX1B, RBP associated molecule 14-1, RP11-535K18.1, SLIM, SLIM-1, SLIMMER, Skeletal muscle LIM-protein 1, XMPMA, bA535K18.1, four-and-a-half Lin11

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FHL1 KO cell line available to order. KO validated by Western blot. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 4 and Insertion of the selection cassette in exon 4.

Key facts

Cell type
HeLa
Form
Liquid
Mutation description
Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 4 and Insertion of the selection cassette in exon 4
Disease
Adenocarcinoma
Concentration
Loading...

Properties

Gene name
FHL1
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot

Quality control

STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

Cell culture

Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female

Handling procedures

Initial handling guidelines

Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.

1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.

Subculture guidelines
  • All seeding densities should be based on cell counts gained by established methods.
  • A guide seeding density of 2x104 cells/cm2 is recommended.
  • Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.

Storage

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-196°C
Appropriate long-term storage conditions
-196°C

Notes

Recommended control: Human wild-type HeLa cell line (ab255928). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.

We will provide viable cells that proliferate on revival.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The FHL1 protein also known as Four and a Half LIM Domains 1 operates mechanically as a multifunctional adaptor protein. Its molecular mass is approximately 35 kDa. This protein is widely expressed in skeletal muscle and heart tissues playing a role in various cellular contexts. FHL1 interacts within the cell cytoplasm and nucleus allowing it to assist in multiple cellular processes.

Biological function summary

FHL1 integrates into diverse cellular mechanisms by engaging in protein-protein interactions. It acts as part of complexes that regulate transcription cell differentiation and development. The involvement of FHL1 in muscle growth and maintenance is significant partly due to its expression pattern. In skeletal muscles it helps adapt muscle response to mechanical stress while in cardiac tissue it participates in muscle contractility and integrity.

Pathways

FHL1 plays important roles in both the muscle differentiation pathway and the MAPK/ERK signaling pathway. It influences the transcriptional regulation essential for muscle cell growth and differentiation. FHL1 interacts with proteins such as MyoD during muscle formation and MEF2 in the MAPK/ERK pathway modulating cellular responses to external signals and stressors.

Associated diseases and disorders

FHL1's involvement is evident in conditions such as Emery-Dreifuss muscular dystrophy and cardiomyopathy. Mutations or dysregulation of this protein can impair muscle function leading to myopathy. It associates with other proteins like emerin in genetic disorders affecting muscle tissues and structural integrity revealing its vital role in maintaining muscular health.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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