FOXM1 KO cell line available to order. Free of charge wild type control provided.
FKHL16, FOXM1B, FOXM1_HUMAN, Forkhead box M1, Forkhead box protein M1, Forkhead-related protein FKHL16, HFH-11, HNF-3/fork-head homolog 11, HNF3, Hepatocyte nuclear factor 3 forkhead homolog 11, M-phase phosphoprotein 2, MPHOSPH2, MPM-2 reactive phosphoprotein 2, PIG29, TGT3, Transcription factor Trident, Trident, WIN, Winged-helix factor from INS-1 cells, forkhead like 16
FOXM1 KO cell line available to order. Free of charge wild type control provided.
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Although we aim to provide customers with a homozygous clone, feasibility will be dependent on the biology of the protein. Should only heterozygous edits be achieved, you will be notified of the outcome and be asked to confirm whether the cell line is acceptable. All clones will be accompanied with DNA sequencing data, and the mutation description.
Recommended control: Human wild-type MCF7 cell line (ab288560). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
FOXM1 also known as Forkhead Box M1 functions mechanically as a transcription factor. This target plays an important role in the regulation of gene expression during the cell cycle. It is characterized by a molecular weight of approximately 85 kDa. FOXM1 is mainly expressed in actively proliferating cells including the liver lungs and thymus tissues linking it with processes involved in cellular growth and division.
The transcription factor FOXM1 regulates the expression of genes important for DNA replication and mitosis. It forms part of a complex network with other proteins to initiate and sustain cell proliferation. This protein regulates key regulatory genes such as Cyclin B1 and Plk1 ensuring proper transition through various phases of the cell cycle. The presence of FOXM1 in normal tissues emphasizes its role in maintaining healthy cell division.
FOXM1 is integral to the regulation of cell cycle pathways and interacts with proteins involved in the PI3K/AKT signaling pathway. This target coordinates with various proteins to facilitate progression through cell cycle checkpoints and modulate responses to growth signals. Proteins such as CDK1 and SKP2 operate alongside FOXM1 to advance cells from G2 phase into mitosis demonstrating its involvement in cell cycle control mechanics.
FOXM1 shows significant association with cancer progression and metastasis. Overexpression of FOXM1 has been observed in colorectal and breast cancers indicating its potential as a therapeutic target. FOXM1 interacts with proteins like β-catenin in cancerous states influencing tumor growth and survival. Mutations and dysregulation of FOXM1 contribute to oncogenic processes further underlining its role in disease development.
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