Human FUK knockout HEK-293T cell line
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FCSK KO cell line available to order. KO validated by. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon 11 and 1 bp deletion in exon 11 and 25 bp deletion in exon 11.
View Alternative Names
1110046B12Rik, FLJ39408, FUK_HUMAN, Fucokinase, L-fucose kinase
- Sanger seq
Unknown
Sanger Sequencing - Human FUK knockout HEK-293T cell line (AB267312)
Allele-3 : 1 bp deletion in exon 11.
- Sanger seq
Unknown
Sanger Sequencing - Human FUK knockout HEK-293T cell line (AB267312)
Allele-1 : 25 bp deletion in exon11
- Sanger seq
Unknown
Sanger Sequencing - Human FUK knockout HEK-293T cell line (AB267312)
Allele-2 : 10 bp deletion in exon 11.
Product details
Recommended control: Human wild-type HEK293T cell line (ab255449). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
FUK plays a role in the salvage pathway of L-fucose metabolism. This pathway recycles free L-fucose into GDP-fucose which becomes essential for glycoprotein and glycolipid synthesis. FUK operates not as part of a larger protein complex but individually facilitating the utilization of fucose derived from dietary sources or breakdown of glycoproteins. The enzyme regulates intracellular concentrations of fucose impacting cell surface glycoconjugates important for cell signaling.
Pathways
FUK integrates into the fucose salvage pathway that links to glycosylation processes. This situates FUK within the larger context of glycan biosynthesis pathways where it intersects with enzymes such as GDP-fucose synthase. These pathways significantly influence the modification and function of proteins and lipids on cell surfaces affecting cellular communication and host-pathogen interactions.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Target data
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com