Human FZD6 (Frizzled 6) knockout HeLa cell line
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FZD6 KO cell line available to order. KO validated by Western blot. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 3 and 2 bp deletion in exon 3 and 5 bp deletion in exon 3.
View Alternative Names
FZD6_HUMAN, Frizzled 6 seven transmembrane spanning receptor, Frizzled drosophila homolog of 6, Frizzled family receptor 6, Frizzled homolog 6, Frizzled homolog 6 (Drosophila), Frizzled-6, Fz-6, Hfz 6, NDNC10
- WB
Lab
Western blot - Human FZD6 (Frizzled 6) knockout HeLa cell line (AB265280)
Lane 1 : Wild-type HeLa cell lysate 30 µg
Lane 2 : FZD6 knockout HeLa cell lysate 30 µg
Lane 3 : HTC116 cell lysate 30 µg
Lane 4 : Daudi cell lysate 30 µg
Lanes 1 - 4 : Merged signal (red and green). Green - anti-Frizzled 6 antibody observed at 70 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55kDa.
Anti-Frizzled 6 antibody was shown to react with Frizzled 6 in wild-type cells in Western blot with loss of signal observed in FZD6 knockout cell line ab265280 (FZD6 knockout cell lysate ab258874). Wild-type and FZD6 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with anti-Frizzled 6 antibody and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
All lanes:
Anti-Frizzled 6 antibody at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 30 µg
Lane 2:
Western blot - Human FZD6 (Frizzled 6) knockout HeLa cell line (ab265280)
Lane 2:
Western blot - Human FZD6 (Frizzled 6) knockout HeLa cell lysate (<a href='/en-us/products/cell-lysates/human-fzd6-frizzled-6-knockout-hela-cell-lysate-ab258874'>ab258874</a>)
Lane 2:
FZD6 knockout HeLa cell lysate at 30 µg
Lane 3:
HTC116 cell lysate at 30 µg
Lane 4:
Daudi cell lysate at 30 µg
Secondary
Lanes 1 - 4:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution
Lanes 1 - 4:
Western blot - Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-680rd-preadsorbed-ab216776'>ab216776</a>) at 1/20000 dilution
Observed band size: 70 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human FZD6 (Frizzled 6) knockout HeLa cell line (AB265280)
Allele-2 : 2 bp deletion in exon 3.
- Sanger seq
Unknown
Sanger Sequencing - Human FZD6 (Frizzled 6) knockout HeLa cell line (AB265280)
Allele-3 : 1 bp deletion in exon 3.
- Sanger seq
Unknown
Sanger Sequencing - Human FZD6 (Frizzled 6) knockout HeLa cell line (AB265280)
Allele-1 : 5 bp deletion in exon 3.
Product details
Recommended control: Human wild-type HeLa cell line (ab255928). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Frizzled 6 acts as a receptor in the Wnt signaling pathway which is important in cellular communication and regulation. It does not function alone; it is part of a complex network involving other Frizzled proteins and Wnt ligands that are key for processes such as cell proliferation differentiation and migration. This signaling is important for maintaining proper cellular responses and is integral to developmental processes.
Pathways
Frizzled 6 integrates into the non-canonical Wnt signaling pathway which diverges from the canonical pathway by not involving β-catenin. In this pathway it cooperates with key proteins like Disheveled (DVL) and forms complexes with Wnt ligands to elicit specific cellular responses. This engagement is essential for tailoring responses to external cellular cues especially in processes like tissue polarity and neural tube closure during embryonic development.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Target data
Complementary Products
Primary Antibodies
AB290728
Anti-Frizzled 6 antibody [EPR25319-149]
BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Frizzled 6 antibody [EPR25319-149] (AB290728)](https://content.abcam.com/products/images/frizzled-6-antibody-epr25319-149-ab290728--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img400885.jpg)
- 0
- 0
Cell Lines & Lysates
AB264750
Human SERPINB5 (MASPIN) knockout HeLa cell line
Advanced Validation
- 0
- 0
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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