GAA KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 7 bp deletion in exon 2 and 8 bp deletion in exon 2.
Images
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 7 bp deletion in exon 2 and 8 bp deletion in exon 2
Alternative names
70 kDa lysosomal alpha-glucosidase, Acid Maltase, Acid alpha glucosidase, Aglucosidase Alfa, Alpha-glucosidase, Glucosidase alpha, Glucosidase alpha acid, Glucosidase alpha acid (Pompe disease glycogen storage disease type II), LYAG_HUMAN, Lysosomal Alpha-Glucosidase
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GAA KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 7 bp deletion in exon 2 and 8 bp deletion in exon 2.
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 7 bp deletion in exon 2 and 8 bp deletion in exon 2
- Concentration
Properties
- Gene name
- GAA
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Handling procedures
- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Cell Culture - Human GAA knockout HEK-293T cell line (ab266259)
Representative images of GAA knockout HEK293T cells, low and high confluency examples (top left and right respectively) and wild-type HEK293T cells, low and high confluency (bottom left and right respectively) showing typical adherent, epithelial-like morphology. Images were captured at 10X magnification using a EVOS XL Core microscope.
Sanger Sequencing - Human GAA knockout HEK-293T cell line (ab266259)
Allele-1: 8 bp deletion in exon2
Sanger Sequencing - Human GAA knockout HEK-293T cell line (ab266259)
Allele-2: 7 bp deletion in exon 2.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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