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GBP2 KO cell line available to order. KO validated by Western blot. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 6.

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Images

Western blot - Human GBP2 knockout A549 cell line (AB267218), expandable thumbnail
  • Cell Culture - Human GBP2 knockout A549 cell line (AB267218), expandable thumbnail
  • Sanger Sequencing - Human GBP2 knockout A549 cell line (AB267218), expandable thumbnail

Key facts

Cell type
A549
Species or organism
Human
Tissue
Lung
Form
Liquid
Knockout validation
Sanger Sequencing, Western blot
Mutation description
Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 6

Alternative names

GTP-binding protein 2, Guanine nucleotide binding protein 2, Guanylate binding protein 2 interferon inducible, HuGBP 2, Interferon induced guanylate binding protein 2

Recommended products

GBP2 KO cell line available to order. KO validated by Western blot. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 6.

Key facts

Cell type
A549
Form
Liquid
Mutation description
Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 6
Disease
Carcinoma
Concentration
Loading...

Properties

Gene name
GBP2
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Zygosity
Homozygous

Quality control

STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

Cell culture

Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male

Handling procedures

Initial handling guidelines

Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.

1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.

Subculture guidelines
  • All seeding densities should be based on cell counts gained by established methods.
  • A guide seeding density of 2x104 cells/cm2 is recommended.
  • Cells should be passaged when they have achieved 80-90% confluence.
  • Do not allow the cell density to exceed 7x104 cells/cm2.
Culture medium
F-12K + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.

Storage

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-196°C
Appropriate long-term storage conditions
-196°C

Notes

Recommended control: Human wild-type A549 cell line (ab255450). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.

We will provide viable cells that proliferate on revival.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

GBP2 also known as Guanylate Binding Protein 2 is a member of the dynamin superfamily of large GTPases. It has an approximate mass of 67 kDa. This protein finds expression predominantly in macrophages and other immune cells. The involvement of GBP2 in cellular defense mechanisms reflects its upregulation by interferon-gamma where it takes part in immune responses against microbial and viral infections.

Biological function summary

Guanylate Binding Protein 2 participates in immune response regulation. It does not function as part of a large multiprotein complex but acts primarily as a monomer. GBP2 disrupts pathogenic molecular machinery by binding to microbial enzymes leading to their inhibition which limits pathogen survival. This mechanism ensures that GBP2 remains a significant factor in the cell's intrinsic ability to fight infections at the molecular level.

Pathways

Guanylate Binding Protein 2 plays an important role in the interferon-induced defense pathways. It participates in the immune effector pathways primarily connecting to processes like the JAK-STAT signaling pathway. Through this involvement GBP2 helps enhance the expression of a variety of immunologically relevant genes. Additionally GBP2 often interacts with other members of the GBP family including GBP1 and GBP5 to amplify immune responses and signaling cascades.

Associated diseases and disorders

Guanylate Binding Protein 2 shows a significant connection to infectious diseases like tuberculosis and viral infections such as hepatitis C. Overexpression or dysregulation of GBP2 can influence the progression and severity of these conditions. Additionally GBP2's interaction with proteins involved in inflammatory responses such as other interferon-induced proteins highlights its influence over auto-inflammatory conditions and its potential as a biomarker for disease progression.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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