GBP3 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 3 and 1 bp insertion in exon 3.
Images
Key facts
- Cell type
- A549
- Species or organism
- Human
- Tissue
- Lung
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 3 and 1 bp insertion in exon 3
Alternative names
DKFZp686E0974, DKFZp686L15228, FLJ10961, GBP3_HUMAN, GTP-binding protein 3, Guanine nucleotide-binding protein 3, Guanylate-binding protein 3, SPG3A, guanylate-binding protein 3 delta C
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GBP3 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 3 and 1 bp insertion in exon 3.
Key facts
- Cell type
- A549
- Species or organism
- Human
- Tissue
- Lung
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 3 and 1 bp insertion in exon 3
- Disease
- Carcinoma
- Concentration
Properties
- Gene name
- GBP3
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 1 US: 1
- Adherent/suspension
- Adherent
- Gender
- Male
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Do not allow the cell density to exceed 7x104 cells/cm2.
- Culture medium
- F-12K + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein known as GBP3 also called Guanylate Binding Protein 3 is a member of the guanylate-binding protein family. This protein has a molecular mass of approximately 67 kDa. It mainly functions in cellular processes involving GTP hydrolysis which plays a significant role in certain immune responses. GBP3 expresses in various tissues including the heart placenta and lungs showing higher presence in immune-related cells and tissues.
- Biological function summary
GBP3 contributes to cellular defense by promoting anti-viral states and mediating immune signaling. It has been found to associate with large protein complexes that enhance its stability and activity during inflammation. This involvement supports various immune functions and contributes to the body's response to pathogenic threats. Such actions highlight GBP3's importance in maintaining cellular immunity and promoting pathogenic resistance.
- Pathways
GBP3 interacts within the interferon-gamma signaling pathway where it facilitates host defense mechanisms. The protein's activity aligns closely with the JAK-STAT pathway important for the transduction of extracellular signals from the environment to the cell nucleus. GBP3 operates alongside other proteins such as GBP1 and GBP5 which are also part of guanylate-binding proteins involved in immune regulation and protection against intracellular bacteria and viruses.
- Associated diseases and disorders
Impairments in GBP3 function have connections to infectious diseases and inflammatory disorders. Studies suggest that altered GBP3 expression might relate to an increased susceptibility to viral infections such as hepatitis. Additionally abnormalities in this protein may associate with chronic inflammatory conditions like Crohn's disease. GBP3 interacts with proteins like STAT1 and GBP1 which play critical roles in modulating immune response and maintaining homeostasis within inflammatory conditions.
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Sanger Sequencing - Human GBP3 knockout A549 cell line (ab267190)
Allele-1: 1 bp insertion in exon3
Sanger Sequencing - Human GBP3 knockout A549 cell line (ab267190)
Allele-2: 1 bp deletion in exon 3.
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