GBP4 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 3 and 2 bp insertion in exon 3.
Images
Key facts
- Cell type
- A549
- Species or organism
- Human
- Tissue
- Lung
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 3 and 2 bp insertion in exon 3
Alternative names
GBP4_HUMAN, GTP-binding protein 4, Guanine nucleotide-binding protein 4, Guanylate-binding protein 4, Mpa2
Recommended products
GBP4 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 3 and 2 bp insertion in exon 3.
Key facts
- Cell type
- A549
- Species or organism
- Human
- Tissue
- Lung
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 3 and 2 bp insertion in exon 3
- Disease
- Carcinoma
- Concentration
Properties
- Gene name
- GBP4
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 1 US: 1
- Adherent/suspension
- Adherent
- Gender
- Male
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Do not allow the cell density to exceed 7x104 cells/cm2.
- Culture medium
- F-12K + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
GBP4 also known as guanylate-binding protein 4 is a member of the guanylate-binding protein family. This family is known for its role in hydrolyzing GTP to GDP and GMP an important mechanical activity that GBP4 performs. GBP4 has a mass of approximately 68 kDa. The protein is expressed in a variety of tissues but it shows higher expression in immune-related cells. It is often upregulated in response to interferon-gamma a cytokine important for immune response modulation.
- Biological function summary
GBP4 plays an important role in immune response against pathogens. By disrupting microbial membranes GBP4 aids in restricting the spread of certain intracellular bacteria. It may also participate in forming a larger complex with other GBP proteins for more efficient pathogen targeting. This action is particularly significant during infections where increased GBP4 expression provides a stronger defense mechanism as part of the innate immune system.
- Pathways
GBP4 integrates functionally into interferon-gamma and JAK-STAT signaling pathways. These pathways are key components of the immune response to infections and inflammation. GBP4's role in these pathways highlights its interaction with other immune proteins such as STAT1 and other guanylate-binding proteins. These interactions highlight GBP4's importance in signaling cascades that strategize cellular immune defenses.
- Associated diseases and disorders
GBP4 exhibits links to conditions like autoimmune diseases and certain infections. In autoimmune pathologies dysregulation involving GBP4 may contribute to inappropriate immune activation potentially related to proteins such as interferon-gamma. Additionally in infections GBP4 plays a role in controlling bacterial infections within macrophages often in cooperation with other members of the guanylate-binding protein family. Understanding these connections provides insights into therapeutic interventions targeting immune pathways in disease management.
Product promise
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2 product images
Sanger Sequencing - Human GBP4 knockout A549 cell line (ab267045)
Allele-2: 2 bp insertion in exon 3.
Sanger Sequencing - Human GBP4 knockout A549 cell line (ab267045)
Allele-1: 1 bp insertion in exon3
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