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GLI1 KO cell line available to order. KO validated by Next Generation Sequencing. Free of charge wild type control provided.

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Next Generation Sequencing - Human GLI1 knockout A549 cell line (AB277815), expandable thumbnail

Key facts

Cell type
A549
Species or organism
Human
Tissue
Lung
Form
Liquid
Knockout validation
Next Generation Sequencing

Alternative names

GLI, GLI Kruppel family member 1, GLI family zinc finger 1, GLI1_HUMAN, Glioma associated oncogene 1, Glioma associated oncogene homolog, Glioma associated oncogene homolog 1 (zinc finger protein), Glioma-associated oncogene, Oncogene GLI, Zfp 5, Zinc finger protein GLI 1

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GLI1 KO cell line available to order. KO validated by Next Generation Sequencing. Free of charge wild type control provided.

Key facts

Cell type
A549
Form
Liquid
Disease
Carcinoma
Concentration
Loading...

Properties

Gene name
GLI1
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Next Generation Sequencing

Quality control

STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

Cell culture

Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male

Handling procedures

Initial handling guidelines

Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.

1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.

Subculture guidelines
  • All seeding densities should be based on cell counts gained by established methods.
  • A guide seeding density of 2x104 cells/cm2 is recommended.
  • Cells should be passaged when they have achieved 80-90% confluence.
  • Do not allow the cell density to exceed 7x104 cells/cm2.
Culture medium
F-12K + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.

Storage

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-196°C
Appropriate long-term storage conditions
-196°C

Notes

Although we aim to provide customers with a homozygous clone, feasibility will be dependent on the biology of the protein. Should only heterozygous edits be achieved, you will be notified of the outcome and be asked to confirm whether the cell line is acceptable. All clones will be accompanied with DNA sequencing data, and the mutation description.

Recommended control: Human wild-type A549 cell line (ab288558). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.

We will provide viable cells that proliferate on revival.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The Gli1 protein also known as GLI family zinc finger 1 acts mechanically as a transcription factor influencing gene expression. It comprises five C2H2-type zinc fingers enabling specific DNA binding activities. Gli1 has a molecular weight of approximately 118 kDa. Expression occurs prominently in skin brain and bone tissues. As an important effector in the Hedgehog (Hh) signaling pathway Gli1 transmits signals from the cell membrane to the nucleus.

Biological function summary

Gli1 functions to regulate cellular proliferation differentiation and survival. As part of the Gli protein family Gli1 operates in concert with other members such as Gli2 and Gli3 though it lacks significant repressor activity unlike some of its counterparts. It acts prominently in developmental processes controlling the expression of genes critical for embryogenesis. Gli1 also maintains stem cell populations and sustains tissue homeostasis in adults particularly in contexts where rapid cell division is necessary like wound healing.

Pathways

Gli1 plays a substantial role in the Hedgehog signaling pathway and interacts dynamically with this system. It receives upstream activation from the Smoothened protein which conveys signals in response to Hedgehog ligand availability. Additionally the Gli1 pathway overlaps with WNT signaling during various developmental stages. Through these interactions Gli1 coordinates tightly with other proteins to effect changes in target gene transcription that influence cell fate decisions.

Associated diseases and disorders

Aberrant Gli1 activity associates strongly with certain cancers such as basal cell carcinoma and medulloblastoma. Persistent activation of the Hedgehog pathway often due to mutations in related proteins like Patched or Smoothened leads to excessive Gli1 activation and subsequent tumorigenesis. Beyond oncology Gli1's dysregulation also connects with congenital disorders such as Gorlin syndrome. Here mutations influence a broad network of genes that often include members of the Gli family causing developmental anomalies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Next Generation Sequencing - Human GLI1 knockout A549 cell line (ab277815), expandable thumbnail

    Next Generation Sequencing - Human GLI1 knockout A549 cell line (ab277815)

    142 bp deletion after Leu588 (edit 1); 63 bp deletion and 1 bp insertion after Leu588 (edit 2)

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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