GNB1 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 2 bp insertion in exon 5 and Insertion of the selection cassette in exon 5.
Images
Key facts
- Cell type
- HCT116
- Species or organism
- Human
- Tissue
- Colon
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 2 bp insertion in exon 5 and Insertion of the selection cassette in exon 5
Alternative names
Beta subunit signal transducing proteins GS/GI, G protein beta 1 subunit, GBB1_HUMAN, Guanine nucleotide binding protein (G protein) beta polypeptide 1, Guanine nucleotide binding protein beta 1 subunit, Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1, Transducin beta chain 1
Recommended products
GNB1 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 2 bp insertion in exon 5 and Insertion of the selection cassette in exon 5.
Key facts
- Cell type
- HCT116
- Species or organism
- Human
- Tissue
- Colon
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 2 bp insertion in exon 5 and Insertion of the selection cassette in exon 5
- Disease
- Carcinoma
- Concentration
Properties
- Gene name
- GNB1
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 1 US: 1
- Adherent/suspension
- Adherent
- Gender
- Male
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- McCoY5a + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The GNB1 protein also known as Guanine nucleotide-binding protein subunit beta-1 is an important component in signal transduction across a variety of systems. With an approximate mass of 37 kDa GNB1 is expressed in numerous tissues throughout the human body including the central nervous system heart and spleen. Its role is to facilitate communication between receptor proteins on the cell surface and the interior machinery of the cell enabling efficient relay of external signals.
- Biological function summary
G-protein signaling inhibitors rely on the interaction of GNB1 within the cellular framework. GNB1 is part of a larger protein complex called heterotrimeric G-proteins. This complex includes three subunits: alpha beta and gamma where GNB1 typically partners with the gamma subunit to stabilize and enhance signal transduction. GNB1 modulates diverse biological processes by enabling proper functioning of neurotransmitter and hormone receptors.
- Pathways
GNB1 plays a role in the GPCR (G-protein-coupled receptor) signaling pathway which is one of the largest and most varied signal transduction pathways. Additionally GNB1 influences the MAPK signaling pathway which is important for regulating processes such as cell proliferation and differentiation. In these pathways GNB1 interacts closely with various proteins such as G-alpha subunits and mitogen-activated proteins ensuring precise and targeted signaling outcomes.
- Associated diseases and disorders
Mutations or dysregulation of GNB1 can lead to significant health issues such as intellectual disability and cancer. GNB1 abnormalities have been linked to an increased susceptibility to neurodevelopmental disorders where its interaction with G-alpha proteins becomes compromised. Additionally irregular GNB1 activity can influence oncogenic pathways contributing to the progression of certain types of cancer by altering cell signaling dynamics.
Product promise
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2 product images
Sanger Sequencing - Human GNB1 knockout HCT116 cell line (ab266890)
Allele-2: Insertion of the selection cassette in exon 5.
Sanger Sequencing - Human GNB1 knockout HCT116 cell line (ab266890)
Allele-1: 2 bp insertion in exon5
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