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GNB2 KO cell line available to order. KO validated by Western blot. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2.

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Images

Western blot - Human GNB2 knockout HEK-293T cell line (AB266528), expandable thumbnail
  • Sanger Sequencing - Human GNB2 knockout HEK-293T cell line (AB266528), expandable thumbnail

Key facts

Cell type
HEK-293T
Species or organism
Human
Tissue
Kidney
Form
Liquid
Knockout validation
Sanger Sequencing, Western blot
Mutation description
Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2

Alternative names

G protein beta 2 subunit, G protein subunit beta-2, GBB2_HUMAN, Gnb2l1, Guanine nucleotide binding protein G I G S G T beta 2 subunit 2, Guanine nucleotide binding protein G protein beta polypeptide 2, Guanine nucleotide binding protein beta 2 subunit, Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-2, OTTHUMP00000174601, OTTHUMP00000174602, Receptor for activated C kinase, Receptor of activated protein kinase C 1, Signal transducing guanine nucleotide binding regulatory protein beta, Transducin beta chain 2

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GNB2 KO cell line available to order. KO validated by Western blot. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2.

Key facts

Cell type
HEK-293T
Form
Liquid
Mutation description
Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2
Concentration
Loading...

Properties

Gene name
GNB2
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Zygosity
Homozygous

Quality control

STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

Cell culture

Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female

Handling procedures

Initial handling guidelines

Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.

1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.

Subculture guidelines
  • All seeding densities should be based on cell counts gained by established methods.
  • A guide seeding density of 2x104 cells/cm2 is recommended.
  • Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.

Storage

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-196°C
Appropriate long-term storage conditions
-196°C

Notes

Recommended control: Human wild-type HEK293T cell line (ab255449). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.

We will provide viable cells that proliferate on revival.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

GNB2 also known as Guanine Nucleotide Binding Protein Subunit Beta 2 is part of the G protein family. It has a molecular mass of approximately 38 kDa. GNB2 functions mechanically by acting as a modulator or transducer in various signaling pathways. This protein is expressed in a wide range of tissues including the brain heart and muscles. Its role involves interacting with alpha and gamma subunits to form a heterotrimeric G protein complex that is important for signal transduction.

Biological function summary

In the context of cellular processes GNB2 is involved in transmitting signals from activated G protein-coupled receptors to different intracellular effectors. It forms an essential part of the G protein complex which plays a role in facilitating the exchange of GDP for GTP on the alpha subunit. The activation of the G protein complex enables downstream signaling that governs multiple physiological responses such as sensory perception and cellular communication.

Pathways

GNB2 is heavily involved in pathways related to signal transduction and sensory perception. It plays a significant role within the GPCR (G protein-coupled receptor) signaling pathway an important pathway involved in transmitting signals from diverse stimuli outside a cell to its interior. In this context GNB2 interacts with related proteins such as GNAI2 contributing to the regulation of ion channels adenylyl cyclases and other effectors. This connectivity enables precise cellular responses to external signals.

Associated diseases and disorders

Research indicates that GNB2 is associated with cardiac arrhythmias and neuropsychiatric disorders. It has connections to other proteins like GNAO1 in the context of these conditions. Mutations or dysregulation of GNB2 can disrupt normal signaling processes leading to these diseases. Understanding the role of GNB2 in these disorders highlights potential therapeutic targets for developing new interventions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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