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GRIN2A KO cell line available now. KO validated by Next Generation Sequencing. Free of charge wild type control available.
- Cell type
U-87 MG
- Species or organism
Human
- Tissue
Brain
- Form
Liquid
- Knockout validation
Next Generation Sequencing
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GRIN2A KO cell line available now. KO validated by Next Generation Sequencing. Free of charge wild type control available.
Key facts
- Cell type
U-87 MG
- Species or organism
Human
- Tissue
Brain
- Form
Liquid
- Knockout validation
Next Generation Sequencing
- Disease
Glioblastoma
- Concentration
Properties
- Gene name
GRIN2A
- Gene editing type
Knockout
- Gene editing method
CRISPR technology
- Knockout validation
Next Generation Sequencing
Cell culture
- Biosafety level
EU: 1 US: 1
- Viability
~ 80%
- Adherent/suspension
Adherent
- Gender
Male
Handling procedures
- Initial handling guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
EMEM + 10% FBS
- Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
Dry Ice
- Appropriate long-term storage conditions
-196°C
Notes
Recommended control: Human wild-type U-87 MG cell line (ab278079). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com