Human GSN (Gelsolin) knockout HeLa cell line
- Advanced Validation
- What is this?
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GSN KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 6 and 20 bp deletion in exon 6. To order both knockout and wild-type control cells: select '2 x 1000000 Cells/vial'. To order only knockout cells: select '1000000 Cells/vial'.
View Alternative Names
ADF, AGEL, Actin-depolymerizing factor, Brevin, DKFZp313L0718, GELS_HUMAN, Gelsolin, Gsn
- WB
Collaborator
Western blot - Human GSN (Gelsolin) knockout HeLa cell line (AB265201)
ab109014 was shown to react with GSN in wild-type HeLa cells in Western blot with loss of signal observed in GSN knockout cell line ab265201 (GSN knockout cell lysate ab257204). Wild-type HeLa and GSN knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab109014 overnight at 4 ° at a 1/20000 dilution. Blots were incubated with goat anti-rabbit HRP secondary antibodies at 1/5000 before imaging. These data were provided by YCharOS Inc. an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.
All lanes:
Western blot - Anti-Gelsolin antibody [EPR1942] (<a href='/en-us/products/primary-antibodies/gelsolin-antibody-epr1942-ab109014'>ab109014</a>) at 1/20000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
GSN knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human GSN (Gelsolin) knockout HeLa cell line (ab265201)
Predicted band size: 86 kDa
false
- WB
Collaborator
Western blot - Human GSN (Gelsolin) knockout HeLa cell line (AB265201)
ab11081 was shown to react with GSN in wild-type HeLa cells in Western blot with loss of signal observed in GSN knockout cell line ab265201 (GSN knockout cell lysate ab257204). Wild-type HeLa and GSN knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab11081 overnight at 4 ° at a 1/200 dilution. Blots were incubated with goat anti-mouse HRP secondary antibodies at 1/5000 before imaging. This data was kindly provided by the YCharOS Inc. an open science company with the mission of characterizing every commercially available antibody reagent. abcam are working with YCharOS to support their mission of antibody characterisation using knockout cell lines.
All lanes:
Western blot - Anti-Gelsolin antibody [GS-2C4] (<a href='/en-us/products/primary-antibodies/gelsolin-antibody-gs-2c4-ab11081'>ab11081</a>) at 1/200 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
GSN knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human GSN (Gelsolin) knockout HeLa cell line (ab265201)
Predicted band size: 86 kDa
false
- WB
Collaborator
Western blot - Human GSN (Gelsolin) knockout HeLa cell line (AB265201)
ab74420 was shown to react with GSN in wild-type HeLa cells in Western blot with loss of signal observed in GSN knockout cell line ab265201 (GSN knockout cell lysate ab257204). Wild-type HeLa and GSN knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab74420 overnight at 4 ° at a 1/20000 dilution. Blots were incubated with goat anti-rabbit HRP secondary antibodies at 1/5000 before imaging. This data was kindly provided by the YCharOS Inc. an open science company with the mission of characterizing every commercially available antibody reagent. abcam are working with YCharOS to support their mission of antibody characterisation using knockout cell lines.
All lanes:
Western blot - Anti-Gelsolin antibody (<a href='/en-us/products/primary-antibodies/gelsolin-antibody-ab74420'>ab74420</a>) at 1/20000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
GSN knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human GSN (Gelsolin) knockout HeLa cell line (ab265201)
Predicted band size: 86 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human GSN (Gelsolin) knockout HeLa cell line (AB265201)
Allele-1 : 20 bp deletion in exon 6.
- Sanger seq
Unknown
Sanger Sequencing - Human GSN (Gelsolin) knockout HeLa cell line (AB265201)
Allele-2 : 1 bp deletion in exon 6.
- Cell Culture
Unknown
Cell Culture - Human GSN (Gelsolin) knockout HeLa cell line (AB265201)
Representative images of GSN knockout HeLa cells, low and high confluency examples (top left and right respectively) and wild-type HeLa cells, low and high confluency (bottom left and right respectively) showing typical adherent, epithelial-like morphology. Images were captured at 10X magnification using a EVOS XL Core microscope.
Reactivity data
Product details
We will provide viable cells that proliferate on revival.
Western blot data indicates that the CRISPR gene edit may have resulted in a truncation of the protein of interest. Please see data images.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
This protein acts in cell movement apoptosis and signal transduction. Gelsolin contributes to the remodeling of the actin cytoskeleton by promoting the disassembly of actin filaments allowing cells to change shape and move. Although it operates independently gelsolin's activity can be influenced by calcium ions and phosphoinositides leading to its participation in various cellular activities. Gelsolin plays a role in platelet activation and may regulate cellular responses through association with the cytoskeletal complex.
Pathways
Gelsolin participates in the regulation of the actin cytoskeleton and apoptotic pathways. Within these pathways gelsolin interacts with proteins like cofilin to modulate actin filament dynamics. In apoptotic pathways it collaborates by altering the cytoskeletal architecture impacting cell death mechanisms. Gelsolin modifications in these pathways have implications for cellular processes like cell migration and apoptosis bringing it into relation with proteins such as caspases.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gelsolin antibody [EPR1942] (AB109014)](https://content.abcam.com/products/images/gelsolin-antibody-epr1942-ab109014--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img72744.jpg)
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com