Human GSTT2B (GSTT2) knockout HeLa cell line
- Advanced Validation
- What is this?
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GSTT2B KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp deletion in exon 2. To order both knockout and wild-type control cells: select '2 x 1000000 Cells/vial'. To order only knockout cells: select '1000000 Cells/vial'.
View Alternative Names
AI266894, EC 2.5.1.18, GST 12 12, GST class-theta-2, GSTT2 protein, GSTT2B, GSTT2P, GSTT2_HUMAN, Glutathione S transferase 12, Glutathione S transferase Yrs Yrs, Glutathione S-transferase theta-2, Glutathione S-transferase theta-2B, MGC182032, OTTHUMP00000198491, OTTHUMP00000198492, Yrs, mGSTT2
- WB
Lab
Western blot - Human GSTT2B (GSTT2) knockout HeLa cell line (AB264703)
Lanes 1-4 : Merged signal (red and green). Green - ab176336 observed at 28 kDa. Red - loading control, ab7291 observed at 50 kDa.
ab176336 Anti-GSTT2 antibody [EPR8136(2)] was shown to specifically react with GSTT2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264703 (knockout cell lysate ab258443) was used. Wild-type and GSTT2 knockout samples were subjected to SDS-PAGE. ab176336 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-GSTT2 antibody [EPR8136(2)] (<a href='/en-us/products/primary-antibodies/gstt2-antibody-epr81362-ab176336'>ab176336</a>) at 1/500 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
GSTT2B knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human GSTT2B (GSTT2) knockout HeLa cell line (ab264703)
Lane 3:
Human lung tissue lysate at 20 µg
Lane 4:
A549 cell lysate at 20 µg
Predicted band size: 28 kDa
Observed band size: 28 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human GSTT2B (GSTT2) knockout HeLa cell line (AB264703)
Homozygous : 2 bp deletion in exon 2.
Reactivity data
Product details
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
GSTT2 facilitates the detoxification process by catalyzing reactions that neutralize harmful compounds. It catalyzes the conjugation of glutathione to form less reactive electrophilic or hydrophobic substances. GSTT2 does not function as part of a larger complex within cells but has an important role in maintaining the redox balance. The enzyme contributes to cellular defense mechanisms ensuring proper cellular functions in stressful environments.
Pathways
The activity of GSTT2 has connections to the glutathione metabolism and drug metabolism pathways. These pathways are important for neutralizing endogenous and exogenous toxins. In the glutathione metabolism pathway GSTT2 works in conjunction with other GST enzymes like GSTP1 and GSTA1 to manage oxidative stress. The protein indirectly supports cellular detoxification processes influencing how effectively a cell can endure and adapt to toxic insults.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Target data
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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