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GSTT2B KO cell line available to order. KO validated by Western blot. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp deletion in exon 2.

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Images

Western blot - Human GSTT2B (GSTT2) knockout HeLa cell line (AB264703), expandable thumbnail
  • Sanger Sequencing - Human GSTT2B (GSTT2) knockout HeLa cell line (AB264703), expandable thumbnail

Key facts

Cell type
HeLa
Species or organism
Human
Tissue
Cervix
Form
Liquid
Knockout validation
Sanger Sequencing, Western blot
Mutation description
Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp deletion in exon 2

Alternative names

AI266894, EC 2.5.1.18, GST 12 12, GST class-theta-2, GSTT2 protein, GSTT2B, GSTT2P, GSTT2_HUMAN, Glutathione S transferase 12, Glutathione S transferase Yrs Yrs, Glutathione S-transferase theta-2, Glutathione S-transferase theta-2B, MGC182032, OTTHUMP00000198491, OTTHUMP00000198492, Yrs, mGSTT2

Recommended products

GSTT2B KO cell line available to order. KO validated by Western blot. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp deletion in exon 2.

Key facts

Cell type
HeLa
Form
Liquid
Mutation description
Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp deletion in exon 2
Disease
Adenocarcinoma
Concentration
Loading...

Properties

Gene name
GSTT2B
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Zygosity
Homozygous

Quality control

STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

Cell culture

Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female

Handling procedures

Initial handling guidelines

Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.

1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.

Subculture guidelines
  • All seeding densities should be based on cell counts gained by established methods.
  • A guide seeding density of 2x104 cells/cm2 is recommended.
  • Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.

Storage

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-196°C
Appropriate long-term storage conditions
-196°C

Notes

Recommended control: Human wild-type HeLa cell line (ab255928). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.

We will provide viable cells that proliferate on revival.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

GSTT2 also known as Glutathione S-transferase Theta-2 belongs to the GST superfamily of enzymes. This protein has an approximate molecular mass of 27 kDa. GSTT2 is involved in the conjugation of reduced glutathione to various electrophilic compounds facilitating the detoxification process. It is expressed in several tissues with notable presence in the liver kidney and testis. GSTT2 plays a role in processing many xenobiotic substances enhancing cell resistance to oxidative stress.

Biological function summary

GSTT2 facilitates the detoxification process by catalyzing reactions that neutralize harmful compounds. It catalyzes the conjugation of glutathione to form less reactive electrophilic or hydrophobic substances. GSTT2 does not function as part of a larger complex within cells but has an important role in maintaining the redox balance. The enzyme contributes to cellular defense mechanisms ensuring proper cellular functions in stressful environments.

Pathways

The activity of GSTT2 has connections to the glutathione metabolism and drug metabolism pathways. These pathways are important for neutralizing endogenous and exogenous toxins. In the glutathione metabolism pathway GSTT2 works in conjunction with other GST enzymes like GSTP1 and GSTA1 to manage oxidative stress. The protein indirectly supports cellular detoxification processes influencing how effectively a cell can endure and adapt to toxic insults.

Associated diseases and disorders

GSTT2's role in detoxification links it to conditions such as cancer and liver diseases. Altered GSTT2 expression has associations with the development of hepatocellular carcinoma. The protein interacts with related GST family members like GSTM1 and GSTP1 which play roles in modulating cancer susceptibility. Due to its role in processing carcinogens and protecting tissues from oxidative damage GSTT2 presents as a noteworthy protein in understanding disease mechanisms and potential therapeutic targets.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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