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AB262478

Human H1F0 knockout A-431 cell line

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H1-0 KO cell line available to order. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control available. To order both knockout and wild-type control cells: select '2 x 1000000 Cells/vial'. To order only knockout cells: select '1000000 Cells/vial'.

View Alternative Names

H1 histone family member 0, H1(0), H10_HUMAN, H1FV, H1f0, Histone H1'', Histone H1(0), Histone H1.0, Histone H10, Histone H5, MGC5241, N-terminally processed

4 Images
Western blot - Human H1F0 knockout A-431 cell line (AB262478)
  • WB

Lab

Western blot - Human H1F0 knockout A-431 cell line (AB262478)

Lanes 1 - 3 : Merged signal (red and green). Green - ab11080 observed at 30 kDa. Red - loading control ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55 kDa.

ab11080 was shown to react with Histone H1 in wild-type A-431 cells in Western blot with loss of signal observed in H1F0 knockout cell line ab262478 (knockout cell lysate ab263919). Wild-type A-431 and H1F0 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab11080 and ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4°C at a 1 in 500 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-Histone H1.0 antibody [27] (<a href='/en-us/products/primary-antibodies/histone-h10-antibody-27-ab11080'>ab11080</a>) at 1/500 dilution

Lane 1:

Wild-type A431 cell lysate at 40 µg

Lane 2:

H1F0 knockout A431 cell lysate at 40 µg

Lane 2:

Western blot - Human H1F0 knockout A-431 cell line (ab262478)

Lane 3:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 40 µg

Predicted band size: 21 kDa

Observed band size: 30 kDa

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Western blot - Human H1F0 knockout A-431 cell line (AB262478)
  • WB

Lab

Western blot - Human H1F0 knockout A-431 cell line (AB262478)

Lanes 1 - 3 : Merged signal (red and green). Green - ab11079 observed at 30 kDa. Red - loading control ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55 kDa.

ab11079 was shown to react with Histone H1 in wild-type A-431 cells in Western blot with loss of signal observed in H1F0 knockout cell line ab262478 (knockout cell lysate ab263919). Wild-type A-431 and H1F0 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab11079 and ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4°C at a 1 in 500 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-Histone H1.0 antibody [34] (<a href='/en-us/products/primary-antibodies/histone-h10-antibody-34-ab11079'>ab11079</a>) at 1/500 dilution

Lane 1:

Wild-type A431 cell lysate at 40 µg

Lane 2:

H1F0 knockout A431 cell lysate at 40 µg

Lane 2:

Western blot - Human H1F0 knockout A-431 cell line (ab262478)

Lane 3:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 40 µg

Predicted band size: 21 kDa

Observed band size: 30 kDa

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Western blot - Human H1F0 knockout A-431 cell line (AB262478)
  • WB

Lab

Western blot - Human H1F0 knockout A-431 cell line (AB262478)

Lanes 1 - 3 : Merged signal (red and green). Green - ab134914 observed at 30 kDa. Red - loading control, ab7291 (Mouse anti-Alpha Tubulin [DM1A] observed at 55kDa.

ab134914 was shown to react with Histone H1.0 in wild-type A-431 cells in Western blot. Loss of signal was observed when H1F0 knockout cell line ab262478 (knockout cell lysate ab263919) was used. Wild-type and H1F0 A-431 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab134914 and ab7291 (Mouse anti-Alpha Tubulin [DM1A] overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Histone H1.0 antibody [EPR6536] (<a href='/en-us/products/primary-antibodies/histone-h10-antibody-epr6536-ab134914'>ab134914</a>) at 1/1000 dilution

Lane 1:

Wild-type A431 cell lysate at 20 µg

Lane 2:

H1F0 knockout A431 cell lysate at 20 µg

Lane 2:

Western blot - Human H1F0 knockout A-431 cell line (ab262478)

Lane 3:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Predicted band size: 21 kDa

Observed band size: 30 kDa

false

Western blot - Human H1F0 knockout A-431 cell line (AB262478)
  • WB

Lab

Western blot - Human H1F0 knockout A-431 cell line (AB262478)

Lanes 1 - 2 : Merged signal (red and green). Green - ab125027 observed at 33 kDa. Red - loading control, ab7291 (Mouse anti-Alpha Tubulin [DM1A] observed at 55kDa.

ab125027 was shown to react with H1F0 in wild-type A-431 cells in Western blot Loss of signal was observed when H1F0 knockout cell line ab262478 (knockout cell lysate ab263919) was used. Wild-type A-431 and H1F0 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab125027 and ab7291 (Mouse anti-Alpha Tubulin [DM1A] overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Histone H1.0 antibody [EPR6537] (<a href='/en-us/products/primary-antibodies/histone-h10-antibody-epr6537-ab125027'>ab125027</a>) at 1/1000 dilution

Lane 1:

Wild-type A431 (Human epidermoid carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

H1F0 knockout A431 (Human epidermoid carcinoma cell line) whole cell lysate at 20 µg

Lane 2:

Western blot - Human H1F0 knockout A-431 cell line (ab262478)

Predicted band size: 21 kDa

Observed band size: 33 kDa

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Key facts

Cell type

A-431

Species or organism

Human

Tissue

Skin

Form

Liquid

form

Knockout validation

Next Generation Sequencing,Western blot

Disease

Epidermoid Carcinoma

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

We will provide viable cells that proliferate on revival.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
H1-0
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Next Generation Sequencing, Western blot
Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-196°C
Appropriate long-term storage conditions
-196°C
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Handling procedures

Initial handling guidelines

Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.

1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.

Subculture guidelines
  • All seeding densities should be based on cell counts gained by established methods.
  • A guide seeding density of 2x104 cells/cm2 is recommended.
  • Cells should be passaged when they have achieved 80-90% confluence.
Culture medium

DMEM (High Glucose) + 10% FBS

Cryopreservation medium

Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.

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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 1 US: 1

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Target data

See full target information H1-0
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Complementary Products

Primary Antibodies

AB134914

Anti-Histone H1.0 antibody [EPR6536]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H1.0 antibody [EPR6536] (AB134914)

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Proteins & Peptides

AB198676

Recombinant Human Histone H1 protein

SDS-PAGE - Recombinant Human Histone H1 protein (AB198676)

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Primary Antibodies

AB125066

Anti-Glutathione Peroxidase 4 antibody [EPNCIR144]

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (AB125066)

  • 5
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Cell Lines & Lysates

AB266883

Human NFKB2 (NFkB p100/NFKB2) knockout HCT116 cell line

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Western blot - Human NFKB2 (NFkB p100/NFKB2) knockout HCT116 cell line (AB266883)

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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