HAO1 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 22 bp deletion in exon 3 and 4 bp deletion in exon 3.
Images
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 22 bp deletion in exon 3 and 4 bp deletion in exon 3
Alternative names
(S) 2 hydroxy acid oxidase, GOX1, Glycolate oxidase, HAO1, HAOX1_HUMAN, Hydroxyacid oxidase 1, MGC142225, MGC142227, OTTHUMP00000030231
Recommended products
HAO1 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 22 bp deletion in exon 3 and 4 bp deletion in exon 3.
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 22 bp deletion in exon 3 and 4 bp deletion in exon 3
- Disease
- Adenocarcinoma
- Concentration
Properties
- Gene name
- HAO1
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The enzyme HAO1 also known as GOX (glycolate oxidase) functions by catalyzing the oxidation of glycolate to glyoxylate generating hydrogen peroxide in peroxisomes. The molecular mass of HAO1 is approximately 43 kDa. It is expressed in several tissues with high levels found in liver and kidney. The protein may also be referred to as alpha GOX which highlights its identity and specialization within oxidase family.
- Biological function summary
HAO1 facilitates the metabolism of hydroxyl acids playing an important role in glyoxylate and dicarboxylate metabolism. It does not form part of a multi-protein complex but operates as a monomer maintaining cellular homeostasis of organic acids. By helping convert glycolate into a form that cells use or excrete HAO1 prevents the accumulation of compounds that can become toxic at elevated levels.
- Pathways
Glycolate oxidase takes part in the glyoxylate cycle and photorespiration in plants which also has parallels in animal metabolism where it participates in handling waste products. HAO1 interacts within the network of metabolic pathways along with proteins like lactate dehydrogenase that facilitate the conversion of chemical compounds. It plays a role in broader metabolomic balancing acts that maintain overall organismal homeostasis.
- Associated diseases and disorders
Mutations and dysfunctions in HAO1 have links to primary hyperoxaluria type 1 a disease characterized by excessive production and build-up of oxalate leading to kidney stones and renal failure. The interplay with proteins like alanine:glyoxylate aminotransferase which helps detoxify glyoxylate is important in these conditions. This disorder highlights the importance of proper glycolate metabolism and its regulation in maintaining health.
Product promise
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2 product images
Sanger Sequencing - Human HAO1 (GOX) knockout HeLa cell line (ab264716)
Allele-1: 22 bp deletion in exon 3.
Sanger Sequencing - Human HAO1 (GOX) knockout HeLa cell line (ab264716)
Allele-2: 4 bp deletion in exon 3.
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