HERC6 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 23 bp deletion in exon 1.
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Key facts
- Cell type
- A549
- Species or organism
- Human
- Tissue
- Lung
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 23 bp deletion in exon 1
Alternative names
EC 6.3.2.-, FLJ20637, HECT and RLD domain containing E3 ubiquitin protein ligase family member 6, HECT domain and RCC1-like domain 6, HECT domain and RCC1-like domain-containing protein 6, HERC6_HUMAN, Hect domain and RLD 6, Potential ubiquitin ligase, Probable E3 ubiquitin-protein ligase HERC6
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HERC6 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 23 bp deletion in exon 1.
Key facts
- Cell type
- A549
- Species or organism
- Human
- Tissue
- Lung
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 23 bp deletion in exon 1
- Disease
- Carcinoma
- Concentration
Properties
- Gene name
- HERC6
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
- Zygosity
- Homozygous
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 1 US: 1
- Adherent/suspension
- Adherent
- Gender
- Male
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Do not allow the cell density to exceed 7x104 cells/cm2.
- Culture medium
- F-12K + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein HERC6 also known as HECT and RLD domain containing E3 ubiquitin protein ligase 6 plays a mechanical role in the ubiquitination process. It is a part of the HERC family of ubiquitin ligases characterized by an E3 ubiquitin-ligase domain. HERC6 has an approximate molecular mass of 119 kDa. This protein is ubiquitously expressed across various tissues with especially high levels found in the spleen lung and testis. Its mechanical role involves tagging proteins for degradation which is an important function in regulating protein levels.
- Biological function summary
HERC6 contributes to the cellular antiviral response. It participates in the conjugation of the ubiquitin-like protein ISG15 (interferon-stimulated gene 15) to target proteins a modification known as ISGylation. This function supports its participation in host defense mechanisms against viral infections. As an enzyme HERC6 controls ISGylation events either independently or as part of larger protein complexes influencing various cellular processes.
- Pathways
The cellular role of HERC6 intersects with the ISGylation pathway. This process involves several enzymes including the E1-activating ISG15 and E2-conjugating enzymes. Additionally HERC6 has connections to the proteasome degradation pathway where ubiquitination marks proteins for breakdown. This protein works in concert with other components like HERC5 another E3 ligase to facilitate antiviral defense through the modification of viral and host proteins.
- Associated diseases and disorders
HERC6 is associated with conditions involving viral infection and immune response. Its dysfunction can potentially impact the efficiency of the immune defense mechanism making the host more susceptible to viruses like influenza or HIV. As it works closely with ISG15 altered expression or activity of HERC6 or ISG15 could play a role in such viral-related diseases. It may also influence cancer development as disruptions in protein degradation pathways are commonly linked with tumor progression highlighting the need for further research into its pathophysiological roles.
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Sanger Sequencing - Human HERC6 knockout A549 cell line (ab267001)
Homozygous: 23 bp deletion in exon1
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