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HNRNPA2B1 KO cell line available to order. KO validated by Western blot. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp deletion in exon 3.

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Images

Western blot - Human HNRNPA2B1 knockout HEK-293T cell line (AB266404), expandable thumbnail
  • Western blot - Human HNRNPA2B1 knockout HEK-293T cell line (AB266404), expandable thumbnail
  • Cell Culture - Human HNRNPA2B1 knockout HEK-293T cell line (AB266404), expandable thumbnail
  • Sanger Sequencing - Human HNRNPA2B1 knockout HEK-293T cell line (AB266404), expandable thumbnail

Key facts

Cell type

HEK-293T

Species or organism

Human

Tissue

Kidney

Form

Liquid

Knockout validation

Sanger Sequencing, Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp deletion in exon 3

Alternative names

Recommended products

HNRNPA2B1 KO cell line available to order. KO validated by Western blot. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp deletion in exon 3.

Key facts

Cell type

HEK-293T

Form

Liquid

Mutation description

Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp deletion in exon 3

Concentration
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Properties

Gene name

HNRNPA2B1

Gene editing type

Knockout

Gene editing method

CRISPR technology

Knockout validation

Sanger Sequencing, Western blot

Zygosity

Homozygous

Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

Handling procedures

Initial handling guidelines

Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.

1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.

Subculture guidelines

  • All seeding densities should be based on cell counts gained by established methods.

  • A guide seeding density of 2x104 cells/cm2 is recommended.

  • Cells should be passaged when they have achieved 80-90% confluence.

Culture medium

DMEM (High Glucose) + 10% FBS

Cryopreservation medium

Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.

Storage

Shipped at conditions

Dry Ice

Appropriate short-term storage conditions

-196°C

Appropriate long-term storage conditions

-196°C

Notes

Recommended control: Human wild-type HEK293T cell line (Human wild-type HEK-293T cell line ab255449). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.

We will provide viable cells that proliferate on revival.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

HnRNP A2B1 sometimes referred to as heterogeneous nuclear ribonucleoprotein A2/B1 is a multifunctional protein involved in RNA binding and processing. It has a molecular mass of about 38-43 kDa. The protein is expressed in various tissues with high expression levels observed in the brain and liver. hnRNP A2B1 participates in a range of RNA-related processes playing a significant role in mRNA metabolism and transport.

Biological function summary

HnRNP A2B1 contributes to alternative splicing RNA stability and translational regulation. This protein typically associates with complex assemblies that manage RNA processing and transport mechanisms. It interacts with other proteins within the heterogeneous nuclear ribonucleoprotein family collaborating in the regulation of mRNA cycling between the nucleus and cytoplasm affecting gene expression patterns.

Pathways

HnRNP A2B1 exhibits critical involvement in the mRNA splicing pathway and RNA transport pathways. It frequently interacts with proteins such as hnRNP A1 and hnRNP C where they collectively influence pre-mRNA processing and splicing dictating proper RNA maturation and cell function. These pathways are important for maintaining cellular homeostasis and responding to cellular signals.

Associated diseases and disorders

HnRNP A2B1 is linked to neurodegenerative diseases and certain cancers. Abnormal expression or mutations of hnRNP A2B1 are associated with Alzheimer's disease implicating the protein in amyloid precursor protein processing. Additionally dysregulation of this protein shows correlation with breast cancer progression. Proteins like tau and other hnRNPs connect with hnRNP A2B1 in these conditions suggesting a significant role in pathological states.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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