Human HNRNPAB knockout HEK-293T cell line
- Advanced Validation
- What is this?
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HNRNPAB KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 5 bp deletion in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
View Alternative Names
ABBP-1, APOBEC1-binding protein 1, Apolipoprotein B mRNA editing enzyme catalytic polypeptide 1- protein 1, FLJ40338, HNRNPAB, Heterogeneous nuclear ribonucleoprotein A/B, ROAA_HUMAN, hnRNP A/B, hnRNP type A/B protein
- WB
Lab
Western blot - Human HNRNPAB knockout HEK-293T cell line (AB266459)
Lanes 1-4 : Merged signal (red and green). Green - ab199724 observed at 48 kDa. Red - loading control ab8245 observed at 36 kDa.
ab199724 Anti-HNRPab antibody [EPR16944] was shown to specifically react with HNRPab in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266459 (knockout cell lysate ab257467) was used. Wild-type and HNRPab knockout samples were subjected to SDS-PAGE. ab199724 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-HNRPAB antibody [EPR16944] (<a href='/en-us/products/primary-antibodies/hnrpab-antibody-epr16944-ab199724'>ab199724</a>) at 1/1000 dilution
Lane 1:
Wild-type HEK293T cell lysate at 20 µg
Lane 2:
HNRNPAB knockout HEK293T cell lysate at 20 µg
Lane 2:
Western blot - Human HNRNPAB knockout HEK-293T cell line (ab266459)
Lane 3:
K-562 cell lysate at 20 µg
Lane 4:
Jurkat cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution
Predicted band size: 36 kDa
Observed band size: 48 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human HNRNPAB knockout HEK-293T cell line (AB266459)
Homozygous : 5 bp deletion in exon 2
Reactivity data
Product details
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
HNRPAB influences the splicing and transport of pre-mRNA impacting the maturation process of mRNA. It collaborates with other hnRNPs and splicing factors to modulate gene expression. The protein sometimes forms part of larger complexes that are essential for RNA processing. This complex helps to stabilize RNA molecules assisting in their proper transport and translation in the cytoplasm.
Pathways
HNRPAB is involved in the regulation of RNA metabolism and processing pathways. It forms a network with other proteins like hnRNP F and hnRNP H impacting gene expression by influencing mRNA stability and availability. In conjunction with these proteins HNRPAB plays a role in the signal transduction pathways affecting cellular responses and adaptations.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Target data
Complementary Products
![Immunocytochemistry/ Immunofluorescence - Anti-HNRPAB antibody [EPR16944] (AB199724)](https://content.abcam.com/products/images/hnrpab-antibody-epr16944-ab199724--immunocytochemistry-immunofluorescence-img42687.jpg)
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com