HTR3D KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 6 and 2 bp insertion in exon 6.
Images
Key facts
- Cell type
- A549
- Species or organism
- Human
- Tissue
- Lung
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 6 and 2 bp insertion in exon 6
Alternative names
5 HT3 D, 5 hydroxytryptamine receptor 3D, 5-hydroxytryptamine receptor 3 subunit D, HTR3D, MGC119636, MGC119637, Serotonin 5-HT-3D receptor, Serotonin receptor 3D
Recommended products
HTR3D KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 6 and 2 bp insertion in exon 6.
Key facts
- Cell type
- A549
- Species or organism
- Human
- Tissue
- Lung
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 6 and 2 bp insertion in exon 6
- Disease
- Carcinoma
- Concentration
Properties
- Gene name
- HTR3D
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 1 US: 1
- Adherent/suspension
- Adherent
- Gender
- Male
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Do not allow the cell density to exceed 7x104 cells/cm2.
- Culture medium
- F-12K + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The 5HT3D Receptor also known as 5-Hydroxytryptamine Receptor 3D is a subtype of serotonin receptor. It is part of the ligand-gated ion channel family. This receptor is involved in mediating fast synaptic transmission in the central and peripheral nervous systems. The 5HT3D Receptor has a molecular mass of approximately 56 kDa. Expression of this receptor occurs in human brains and the gastrointestinal tract where it performs its role in neuronal signaling.
- Biological function summary
The 5HT3D Receptor functions as a component of the ligand-gated ion channel complex allowing the passage of cations including sodium (Na+) and calcium (Ca2+) upon serotonin binding. This action affects neuronal excitability and neurotransmitter release. The receptor plays a significant role in intestinal motility modulating gut motility and in the central nervous system where it contributes to emotions and cognitive processes.
- Pathways
The 5HT3D Receptor is involved in the serotonergic synapse pathway which is important for transmitting signals in the nervous system. The receptor works alongside other serotonin receptors such as 5HT3A influencing neurotransmitter release and neuronal excitability. It also interacts within the GABAergic synapse pathway where it may affect inhibitory neurotransmission alongside the GABA receptors. These interactions can impact mood regulation and cognitive functions.
- Associated diseases and disorders
The 5HT3D Receptor has associations with conditions such as irritable bowel syndrome (IBS) and anxiety disorders. Abnormal expression or function of this receptor can disrupt gastrointestinal motility leading to IBS symptoms. In the context of anxiety disorders the receptor’s interaction with neurotransmitter pathways can influence anxiety levels. Additionally it connects with other proteins such as serotonin transporter (SERT) and GABA receptors which are implicated in anxiety regulation.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Sanger Sequencing - Human HTR3D (5HT3D Receptor) knockout A549 cell line (ab266992)
Allele-2: 2 bp insertion in exon 6.
Sanger Sequencing - Human HTR3D (5HT3D Receptor) knockout A549 cell line (ab266992)
Allele-1: 1 bp deletion in exon6
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com