IDH3G KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 2.
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Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 2
Alternative names
H IDHG, IDH gamma, IDH3G_HUMAN, Isocitrate dehydrogenase 3 (NAD+) gamma, Isocitrate dehydrogenase [NAD] subunit gamma, Isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial, Isocitric dehydrogenase, Isocitric dehydrogenase subunit gamma, NAD (H) specific isocitrate dehydrogenase gamma subunit, NAD(+) specific ICDH, NAD(+)-specific ICDH subunit gamma, NAD+ specific ICDH, OTTHUMP00000025984, OTTHUMP00000025985, OTTHUMP00000025987, OTTHUMP00000025988, OTTHUMP00000214764, mitochondrial
Recommended products
IDH3G KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 2.
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 2
- Concentration
Properties
- Gene name
- IDH3G
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
- Zygosity
- Homozygous
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The IDH3G protein also called isocitrate dehydrogenase 3 (NAD+) gamma is a subunit of the IDH3 enzyme complex. This protein has a molecular mass of approximately 39 kDa. It is highly expressed in tissues with a high energy demand like muscle cells and neurons. IDH3G plays an important role in the mitochondrial matrix where it catalyzes the oxidative decarboxylation of isocitrate to alpha-ketoglutarate producing NADH and CO2 as byproducts.
- Biological function summary
IDH3G is part of the IDH3 enzyme complex which contains multiple subunits and functions within mitochondria. This complex plays a critical role in the tricarboxylic acid (TCA) cycle also known as the Krebs cycle. This cycle is fundamental for energy production as it supplies reducing equivalents in the form of NADH which drive ATP synthesis through oxidative phosphorylation. The proper functioning of IDH3G contributes to efficient cellular respiration and energy homeostasis.
- Pathways
The function of IDH3G connects to the TCA cycle and the energy metabolism pathway. Within the TCA cycle IDH3G's enzymatic activity ensures the continuation of the cycle and maintains the production of NADH. This is key for the generation of ATP in oxidative phosphorylation. The IDH3 complex works closely with the other components of the TCA cycle including succinate dehydrogenase and citrate synthase ensuring the continuity and regulation of metabolic flux in energy production pathways.
- Associated diseases and disorders
Defects or alterations in IDH3G are associated with metabolic disorders such as lactic acidosis and mitochondrial diseases. These conditions can result from impaired energy production and accumulation of metabolic intermediates. Furthermore IDH3G connections with related proteins could affect cell proliferation and survival in neurodegenerative diseases. Proper regulation and function of IDH3G may impact these diseases through its influence on energy metabolism pathways.
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1 product image
Sanger Sequencing - Human IDH3G knockout HEK-293T cell line (ab266640)
Homozygous: Insertion of the selection cassette in exon2
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