Human IFI16 knockout Jurkat cell line
Be the first to review this product! Submit a review
|
(0 Publication)
IFI16 KO cell line available to order. KO validated by Next Generation Sequencing. Free of charge wild type control available. Knockout achieved by CRISPR/Cas9; X = 4 bp deletion, 2 bp insertion; Frameshift: 100%. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
View Alternative Names
IFI4_MOUSE, Ifi-204, Interferon-activable protein 204, Interferon-inducible protein p204
- NGS
Supplier Data
Next Generation Sequencing - Human IFI16 knockout Jurkat cell line (AB273854)
Knockout achieved by CRISPR/Cas9; X = 4 bp deletion, 2 bp insertion; Frameshift : 100%
Reactivity data
Product details
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water for bath approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method. Based on cell count, seed cells in an appropriate cell culture flask at a density of 2x105 cells/mL. Seeding density is given as a guide only and should be scaled to align with individual lab schedules.
4. Incubate the culture at 37°C incubator with 5% CO2. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x105 cells/mL is recommended.
- Do not allow cell density to exceed 3x106 cells/mL.
Culture medium
RPMI + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Interferon-activated gene 204 plays a role in the host defense mechanism. It functions as part of the cellular response to viral infections and potentially some bacterial infections. The protein is part of a larger complex involved in DNA sensing and it activates immune signaling pathways upon detecting foreign DNA. It interacts with components of the inflammasome participating in activating inflammatory responses necessary for pathogen clearance.
Pathways
Interferon-activated gene 204 participates in the cGAS-STING DNA sensing pathway. This pathway is essential for the production of type I interferons as a defense against viruses. The protein associates with cGAS and STING key players in the recognition of cytosolic DNA. Its role in this pathway highlights its importance in the body's first line of defense closely linking it to the activation of downstream signaling that involves IRF3 and NF-kB.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Suspension
Gender
Male
Target data
Complementary Products
Primary Antibodies
AB32072
Anti-c-Myc antibody [Y69] - ChIP Grade
BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myc antibody [Y69] - ChIP Grade (AB32072)](https://content.abcam.com/products/images/c-myc-antibody-y69-chip-grade-ab32072--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img80839.jpg)
- 4
- 56
Primary Antibodies
AB307201
Anti-Ifi204 antibody [EPR25256-211]
BOND RX™ Validated|20ul selling size|RabMAb|Recombinant
![Immunohistochemistry - Anti-Ifi204 antibody [EPR25256-211] (AB307201)](https://content.abcam.com/products/images/ifi204-antibody-epr25256-211-ab307201--immunohistochemistry-img405035.jpg)
- 0
- 0
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com