IFI16 KO cell line available to order. KO validated by Next Generation Sequencing. Free of charge wild type control provided. Knockout achieved by CRISPR/Cas9; X = 4 bp deletion, 2 bp insertion; Frameshift: 100%.
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Key facts
- Cell type
- Jurkat
- Species or organism
- Human
- Tissue
- Blood
- Form
- Liquid
- Knockout validation
- Next Generation Sequencing
- Mutation description
- Knockout achieved by CRISPR/Cas9; X = 4 bp deletion, 2 bp insertion; Frameshift: 100%
Alternative names
IFI4_MOUSE, Ifi-204, Interferon-activable protein 204, Interferon-inducible protein p204
Recommended products
IFI16 KO cell line available to order. KO validated by Next Generation Sequencing. Free of charge wild type control provided. Knockout achieved by CRISPR/Cas9; X = 4 bp deletion, 2 bp insertion; Frameshift: 100%.
Key facts
- Cell type
- Jurkat
- Species or organism
- Human
- Tissue
- Blood
- Form
- Liquid
- Knockout validation
- Next Generation Sequencing
- Mutation description
- Knockout achieved by CRISPR/Cas9; X = 4 bp deletion, 2 bp insertion; Frameshift: 100%
- Disease
- Non-Hodgkin Lymphoma
- Concentration
Properties
- Gene name
- IFI16
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Next Generation Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 1 US: 1
- Adherent/suspension
- Suspension
- Gender
- Male
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water for bath approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method. Based on cell count, seed cells in an appropriate cell culture flask at a density of 2x105 cells/mL. Seeding density is given as a guide only and should be scaled to align with individual lab schedules.
4. Incubate the culture at 37°C incubator with 5% CO2. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x105 cells/mL is recommended.
- Do not allow cell density to exceed 3x106 cells/mL.
- Culture medium
- RPMI + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- -196°C, -80°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Ifi204 also known as Interferon-activated gene 204 is a protein of the mouse involved in the immune response. It has a molecular mass of approximately 74 kilodaltons. This protein is expressed mainly in activated macrophages and neutrophils and contributes to the innate immune mechanism. It detects cytosolic DNA and activates the STING pathway to induce type I interferon production. It contains a HIN-200 domain that helps in recognizing specific DNA sequences.
- Biological function summary
Interferon-activated gene 204 plays a role in the host defense mechanism. It functions as part of the cellular response to viral infections and potentially some bacterial infections. The protein is part of a larger complex involved in DNA sensing and it activates immune signaling pathways upon detecting foreign DNA. It interacts with components of the inflammasome participating in activating inflammatory responses necessary for pathogen clearance.
- Pathways
Interferon-activated gene 204 participates in the cGAS-STING DNA sensing pathway. This pathway is essential for the production of type I interferons as a defense against viruses. The protein associates with cGAS and STING key players in the recognition of cytosolic DNA. Its role in this pathway highlights its importance in the body's first line of defense closely linking it to the activation of downstream signaling that involves IRF3 and NF-kB.
- Associated diseases and disorders
Altered expression or function of Interferon-activated gene 204 has connections with autoimmunity and inflammatory diseases. Its dysregulation may lead to excessive inflammation contributing to conditions like systemic lupus erythematosus. In this context it interacts with proteins such as AIM2 which is part of the inflammasome complex involved in autoimmune inflammation. Understanding its precise role in these diseases could potentially lead to new therapeutic strategies for managing autoimmune disorders.
Product promise
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Next Generation Sequencing - Human IFI16 knockout Jurkat cell line (ab273854)
Knockout achieved by CRISPR/Cas9; X = 4 bp deletion, 2 bp insertion; Frameshift: 100%
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