Human IFIT5 knockout A549 cell line
Be the first to review this product! Submit a review
|
(0 Publication)
IFIT5 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon 2 and 11 bp deletion in exon 2 and 13 bp deletion in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
View Alternative Names
FLJ53857, FLJ92678, IFIT5_HUMAN, Interferon-induced protein with tetratricopeptide repeats 5, RI58, Retinoic acid and interferon inducible protein (58kD), Retinoic acid- and interferon-inducible 58 kDa protein
- Sanger seq
Unknown
Sanger Sequencing - Human IFIT5 knockout A549 cell line (AB267022)
Allele-2 : 11 bp deletion in exon 2.
- Sanger seq
Unknown
Sanger Sequencing - Human IFIT5 knockout A549 cell line (AB267022)
Allele-1 : 13 bp deletion in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human IFIT5 knockout A549 cell line (AB267022)
Allele-3 : 10 bp deletion in exon 2.
Product details
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Do not allow the cell density to exceed 7x104 cells/cm2.
Culture medium
F-12K + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
IFIT5 is involved in antiviral defense and immune response regulation. It does not function as part of a multi-protein complex but interacts with various signaling molecules to amplify immune responses. By selectively binding to foreign RNA IFIT5 can inhibit viral replication and modulate type I interferon signaling pathways. It plays a significant role in restricting viral protein synthesis giving the host organism time to mount an adaptive immune response.
Pathways
IFIT5 is closely associated with the interferon signaling pathway and the innate immune response pathway. Through its interactions it affects the Jak-STAT signaling pathway which is important for the induction of antiviral states in cells. IFIT5 also has functional links with other proteins such as RIG-I an important sensor in the detection of viral RNAs. Together they provide a robust early immune defense by facilitating cytokine production and ensuring rapid response to infections.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Target data
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com