IFITM1 KO cell line available to order. Free of charge wild type control provided.
Sep-27, CD 225, CD 225 antigen, DSPA2a, Dispanin subfamily A member 2a, IFI 17, IFM1_HUMAN, Interferon-induced protein 17, Interferon-induced transmembrane protein 1, Interferon-inducible protein 9-27, Leu 13, Leu-13 antigen, interferon induced transmembrane protein 1 (9-27)
IFITM1 KO cell line available to order. Free of charge wild type control provided.
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Although we aim to provide customers with a homozygous clone, feasibility will be dependent on the biology of the protein. Should only heterozygous edits be achieved, you will be notified of the outcome and be asked to confirm whether the cell line is acceptable. All clones will be accompanied with DNA sequencing data, and the mutation description.
Recommended control: Human wild-type MCF7 cell line (Human wild-type MCF7 cell line ab288560). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
IFITM1 also known as Interferon-Induced Transmembrane Protein 1 has a molecular weight of approximately 14 kDa. It is a transmembrane protein that plays an important role in host defense mechanisms. IFITM1 is expressed in various tissues including the respiratory tract gastrointestinal tract and the immune system where it serves in defending against pathogenic infections. The protein is usually found on the cell membrane and becomes highly expressed in response to interferon signaling.
This protein inhibits viral entry by restricting the fusion of viral membranes with host cell membranes. IFITM1 does not function alone; it is part of the IFITM family which includes IFITM2 and IFITM3 each contributing to antiviral defense. The protein is strategically positioned to prevent the spread of viruses by blocking them from entering new cells which is important for managing viral propagation.
IFITM1 engages the innate immune response pathway responding to viral infections by acting as a barrier to viral entry. It works alongside related proteins such as IFITM2 and IFITM3 within this pathway. IFITM1 is also part of the interferon pathway where its expression is upregulated in response to interferon signals. This relationship highlights its role in the immune response acting as a frontline protector against the invasion of viruses like influenza and others.
IFITM1 has a significant role in the context of viral infections and certain cancers. It is linked to resistance to viral infections such as influenza showing its importance in antiviral defense. In cancer aberrant expression of IFITM1 is observed in various types including breast and colorectal cancers impacting tumor progression and metastasis. The relationship of IFITM1 with interferon signaling highlights potential therapeutic targets where modulating its activity could influence disease outcomes.
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