INPP5K KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 5 and 32 bp insertion in exon 5.
Images
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 5 and 32 bp insertion in exon 5
Alternative names
43 kDa form skeletal muscle and kidney enriched inositol phosphatase, INP5K_HUMAN, INPP5K, Inositol polyphosphate 5-phosphatase K, PPS, Skeletal muscle and kidney-enriched inositol phosphatase, muscle and kidney-enriched inositol phosphatase
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INPP5K KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 5 and 32 bp insertion in exon 5.
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 5 and 32 bp insertion in exon 5
- Disease
- Adenocarcinoma
- Concentration
Properties
- Gene name
- INPP5K
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SKIP also known as Splicing Factor Kinase Interacting Protein is a protein with a mass of approximately 45 kDa. It functions mechanically as a transcriptional co-regulator and is critical in the regulation of gene expression. SKIP interacts with various transcription factors aiding in RNA polymerase II's activity. SKIP expression is widespread in multiple tissues including liver and brain with higher expression noted in differentiated cells. It plays an essential role in modulating different signaling pathways by interacting with specific proteins.
- Biological function summary
This protein serves multiple roles within the cellular environment. It participates in mRNA splicing as part of the spliceosome complex which is vital for removing introns from pre-mRNA. SKIP modulates the alternative splicing of pre-mRNA thereby affecting the type and function of mRNA that is translated into proteins. Besides its role in splicing SKIP also interacts with other proteins like transcription factors and components of the chromatin remodeling complex. These interactions demonstrate its importance in regulating transcriptional and post-transcriptional processes.
- Pathways
SKIP plays integral roles in pathways such as the Wnt signaling pathway and TGF-beta signaling. These pathways regulate various processes including cell proliferation and differentiation. In the Wnt pathway SKIP interacts with proteins such as beta-catenin influencing gene transcription. It affects the stability of transcription complexes by interacting with factors in these pathways ensuring appropriate cellular responses. These interactions highlight SKIP's contribution to maintaining cellular homeostasis and influencing phenotypic outcomes.
- Associated diseases and disorders
Research links SKIP to cancer and neurodegenerative diseases. SKIP dysregulation has associations with cancer development through its impact on the Wnt pathway affecting cell growth and migration. It interacts with proteins like beta-catenin in this context which is often implicated in oncogenesis. SKIP's role in neurodegenerative diseases such as Alzheimer's involves its influence on the alternative splicing of key neuronal proteins. Incorrect splicing can lead to the production of dysfunctional proteins contributing to disease progression. Hence understanding SKIP's regulation and interactions is essential in targeting these pathologies.
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2 product images
Sanger Sequencing - Human INPP5K (SKIP) knockout HeLa cell line (ab265311)
Allele-1: 1 bp insertion in exon 5.
Sanger Sequencing - Human INPP5K (SKIP) knockout HeLa cell line (ab265311)
Allele-2: 32 bp insertion in exon 5.
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