ITGA11 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 13 and 7 bp deletion in exon 13.
Images
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 13 and 7 bp deletion in exon 13
Alternative names
ITA11_HUMAN, Integrin alpha-11, integrin a11
Recommended products
ITGA11 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 13 and 7 bp deletion in exon 13.
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 13 and 7 bp deletion in exon 13
- Disease
- Adenocarcinoma
- Concentration
Properties
- Gene name
- ITGA11
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ITGA11 also known as Integrin alpha-11 functions mechanically as an alpha subunit that combines with the beta-1 subunit to form a collagen receptor integrin complex. The protein has a mass of approximately 135 kDa and its expression occurs mainly in fibroblastic cells located within connective tissue including areas like skin and lungs. ITGA11 plays a role in cell adhesion helping mediate interactions between cells and the extracellular matrix which is essential for tissue structure and function.
- Biological function summary
ITGA11 influences various cellular processes including cell migration proliferation and differentiation. The protein is part of the alpha-11 beta-1 integrin complex that specifically binds to interstitial collagens. This interaction allows ITGA11 to facilitate communication signals that control cellular behavior. Its effective function in collagen recognition establishes ITGA11 as important for maintaining the structural integrity and repair of tissues particularly during injury or developmental processes.
- Pathways
ITGA11 integrates into the collagen-mediated signaling pathway which connects to cellular responses to the extracellular matrix. In particular ITGA11 interacts with other proteins like integrin beta-1 to affect cell survival and movement. Another important pathway involving ITGA11 is the TGF-beta signaling pathway where it contributes to matrix reorganization and fibroblast activation both significant in tissue development and repair mechanisms.
- Associated diseases and disorders
ITGA11 shows association with fibrotic diseases and certain cancers. In fibrosis excessive collagen deposition occurs and ITGA11's interaction with collagen and its regulatory role in fibroblasts can exacerbate this condition. This relationship links ITGA11 to proteins like integrin beta-1 and TGF-beta which are involved in fibrotic tissue transformation. In cancer ITGA11 supports tumor stroma formation contributing to cancer cell survival and spread emphasizing its importance in tumor biology and as a potential therapeutic target.
Product promise
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Terms & Conditions.
2 product images
Sanger Sequencing - Human ITGA11 knockout HeLa cell line (ab265494)
Allele-2: 1 bp insertion in exon 13.
Sanger Sequencing - Human ITGA11 knockout HeLa cell line (ab265494)
Allele-1: 7 bp deletion in exon 13.
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