Human JAG2 (Jagged 2) knockout HeLa cell line
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JAG2 KO cell line available to order. KO validated by. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 20 bp deletion in exon 12 and 40 bp deletion in exon 12 and 4 bp deletion in exon 12.
View Alternative Names
D12Ggc2e, HJ 2, Jagged 2 precursor, Jagged2, Protein jagged 2, SER2, Syndactylism
- Sanger seq
Unknown
Sanger Sequencing - Human JAG2 (Jagged 2) knockout HeLa cell line (AB265966)
Allele-3 : 4 bp deletion in exon 12.
- Sanger seq
Unknown
Sanger Sequencing - Human JAG2 (Jagged 2) knockout HeLa cell line (AB265966)
Allele-2 : 20 bp deletion in exon 12.
- Sanger seq
Unknown
Sanger Sequencing - Human JAG2 (Jagged 2) knockout HeLa cell line (AB265966)
Allele-1 : 40 bp deletion in exon 12.
- WB
Supplier Data
Western blot - Human JAG2 (Jagged 2) knockout HeLa cell line (AB265966)
Blocking and diluting buffer and concentration : 5% NFDM/TBST.
In Western blot, ab322107 was shown to bind specifically to JAG2. Target of interest was observed at 180 kDa in wild-type Hela cell lysates (lane 1) with no signal observed at this size in JAR2 knockout cell line (lane 2) (lane 2, knockout cell line ab265966.
To minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.
The identity of the bands between 30 kDa and 40 kDa are unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
All lanes:
Western blot - Anti-Jagged 2/JAG2 antibody [EPR29026-90] (<a href='/en-us/products/primary-antibodies/jagged-2-jag2-antibody-epr29026-90-ab322107'>ab322107</a>) at 1/1000 dilution
Lane 1:
HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 60 µg
Lane 2:
Western blot - Human JAG2 (Jagged 2) knockout HeLa cell line (ab265966) at 60 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Observed band size: 180 kDa,36 kDa
false
Exposure time: 26s
Product details
Recommended control: Human wild-type HeLa cell line (ab255928). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The JAG2 protein plays a significant role in development and differentiation processes. It acts as a ligand in the Notch signaling pathway which includes other ligands like Jagged 1 (JAG1) and Delta-like proteins. In this complex JAG2 modulates neurogenesis myogenesis and angiogenesis. By affecting these processes JAG2 contributes to tissue homeostasis and the regulation of stem cells.
Pathways
The Notch signaling pathway involving JAG2 plays an important role in cell-cell communication. JAG2 activity in this pathway impact pathways like T-cell development and bone remodeling. It interacts with proteins like Notch1 and RBP-Jκ forming a pivotal part of the regulatory circuit that manages cell differentiation and proliferation.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Target data
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com