JMJD6 KO cell line available now. Free of charge wild type control available.
PC-3
Human
Prostate
Liquid
Apoptotic cell clearance receptor, Bifunctional arginine demethylase and lysyl-hydroxylase JMJD6, Histone arginine demethylase JMJD6, JMJD6_HUMAN, JmjC domain-containing protein 6, Jumonji domain containing 6, Jumonji domain-containing protein 6, KIAA0585, Lysyl-hydroxylase JMJD6, PSR, PTDSR, PTDSR 1, Peptide-lysine 5-dioxygenase JMJD6, Phosphatidylserine receptor, Protein PTDSR
JMJD6 KO cell line available now. Free of charge wild type control available.
Apoptotic cell clearance receptor, Bifunctional arginine demethylase and lysyl-hydroxylase JMJD6, Histone arginine demethylase JMJD6, JMJD6_HUMAN, JmjC domain-containing protein 6, Jumonji domain containing 6, Jumonji domain-containing protein 6, KIAA0585, Lysyl-hydroxylase JMJD6, PSR, PTDSR, PTDSR 1, Peptide-lysine 5-dioxygenase JMJD6, Phosphatidylserine receptor, Protein PTDSR
PC-3
Human
Prostate
Liquid
Adenocarcinoma
JMJD6
Knockout
CRISPR technology
EU: 1 US: 1
Adherent
Male
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
F-12K + 10% FBS
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Dry Ice
-196°C
Although we aim to provide customers with a homozygous clone, feasibility will be dependent on the biology of the protein. Should only heterozygous edits be achieved, you will be notified of the outcome and be asked to confirm whether the cell line is acceptable. All clones will be accompanied with DNA sequencing data, and the mutation description.
Recommended control: Human wild-type PC-3 cell line (Human wild-type PC-3 cell line ab290718). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
This supplementary information is collated from multiple sources and compiled automatically.
JMJD6 also known as Jumonji Domain Containing 6 or PSR (Phosphatidylserine Receptor) is a protein with a molecular mass of approximately 47 kDa. It functions mechanically as a 2-oxoglutarate- and Fe(II)-dependent oxygenase. This protein resides in the nucleus and cytoplasm with a higher expression in the thymus kidney heart and testis. JMJD6 gets involved in demethylating histones and splicing factors playing a role in gene regulation.
JMJD6 influences gene expression and mRNA splicing processes. It participates in histone arginine demethylation affecting chromatin structure and transcriptional activity. JMJD6 is not known to be part of a larger protein complex but interacts with RNA splicing factors and chromatin modifiers. Its demethylase activity can alter the epigenetic landscape which makes it a vital part of cellular response and differentiation processes.
JMJD6 is linked to the regulation of gene expression and RNA processing pathways. It modulates the transcriptional networks by its demethylase activity affecting how genes are turned on or off. JMJD6 often associates with nuclear receptors and splicing proteins such as SF3B1 integrating within the larger network of gene expression controllers and RNA splicing machinery. These interactions place JMJD6 at a critical juncture in gene expression regulation and RNA maturation.
JMJD6 has connections with cancer and autoimmune diseases. Its role in gene regulation and splicing implicates it in cancer progression often in link with prostate cancer. Abnormal JMJD6 expression can lead to disrupted gene patterns contributing to tumor growth. Additionally its alteration has associations with autoimmune diseases where its interaction with histone-modifying proteins like HDAC affects the immune response. These relationships highlight JMJD6's involvement in disease states marking it as a target of interest in research for therapeutic interventions.
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Terms & Conditions.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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