JUND KO cell line available to order. Free of charge wild type control provided.
AP-1, Activator protein 1, JUND_HUMAN, JunD FL isoform, Transcription factor jun-D, jun D proto oncogene
JUND KO cell line available to order. Free of charge wild type control provided.
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Although we aim to provide customers with a homozygous clone, feasibility will be dependent on the biology of the protein. Should only heterozygous edits be achieved, you will be notified of the outcome and be asked to confirm whether the cell line is acceptable. All clones will be accompanied with DNA sequencing data, and the mutation description.
Recommended control: Human wild-type A549 cell line (Human wild-type A549 cell line ab288558). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
The JunD transcription factor also known as JunD protein belongs to the AP-1 family of transcription factors. It has a mass of approximately 39 kDa. JunD primarily acts by forming dimers with other proteins such as c-Fos and c-Jun. These dimers bind to specific DNA sequences regulating gene expression. JunD shows expression in various tissues but is more abundant in non-dividing and differentiated cells where it helps modulate specific cellular processes.
JunD plays a significant role by influencing cellular proliferation and differentiation. It is often part of a multiprotein complex which can include members of the Fos family and other Jun proteins. These complexes interact with DNA to control the transcription of genes involved in cell cycle regulation apoptosis and stress responses. Different stimuli can alter the composition of these complexes highlighting JunD's adaptability in cellular signaling.
JunD interacts within the MAPK signaling pathway and the oxidative stress response pathway. In the MAPK pathway it can interact with proteins such as ERK and JNK which are critical mediators of cellular responses to growth factors and stress. In the oxidative stress response JunD regulates genes that protect cells from oxidative damage showing its influence on maintaining cellular homeostasis. Other proteins like ATF2 may also interact with JunD within these pathways contributing to transcriptional regulation.
JunD has connections to cancer and neurodegenerative diseases. Its role in regulating cell proliferation links it to tumorigenesis where alterations in its function can lead to uncontrolled cell growth. Additionally in neurodegenerative disorders JunD's influence on oxidative stress response genes may impact disease progression. Furthermore JunD's interaction with the protein Bcl2 associates it with the protection of cells from apoptotic signals illustrating its potential impact on disease development and progression.
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