Human JUP (gamma Catenin) knockout HEK-293T cell line
- Advanced Validation
- What is this?
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JUP KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
View Alternative Names
ARVD 12, CTNNG, Catenin (cadherin associated protein), gamma 80kDa, Catenin gamma, DP 3, DP III, Desmoplakin III, Desmoplakin-3, JUP, Junction plakoglobin, OTTHUMP00000164732, OTTHUMP00000164735, OTTHUMP00000164738, PDGB, PKGB, PLAKOGLOBIN, PLAK_HUMAN, catenin (cadherin-associated protein) gamma (80kD), gamma catenin
- WB
Lab
Western blot - Human JUP (gamma Catenin) knockout HEK-293T cell line (AB266272)
Lanes 1-4 : Merged signal (red and green). Green - ab184919 observed at 82 kDa. Red - loading control ab8245 observed at 37 kDa.
ab184919 Anti-gamma Catenin antibody [EPR17310] was shown to specifically react with gamma Catenin in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266272 (knockout cell lysate ab257269) was used. Wild-type and gamma Catenin knockout samples were subjected to SDS-PAGE. ab184919 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4° at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-gamma Catenin antibody [EPR17310] (<a href='/en-us/products/primary-antibodies/gamma-catenin-antibody-epr17310-ab184919'>ab184919</a>) at 1/1000 dilution
Lane 1:
Wild-type HEK-293T cell lysate at 20 µg
Lane 2:
JUZ knockout HEK-293T cell lysate at 20 µg
Lane 2:
Western blot - Human JUP (gamma Catenin) knockout HEK-293T cell line (ab266272)
Lane 3:
A431 cell lysate at 20 µg
Lane 4:
THP-1 cell lysate at 20 µg
Predicted band size: 82 kDa
Observed band size: 82 kDa
false
- Cell Culture
Unknown
Cell Culture - Human JUP (gamma Catenin) knockout HEK-293T cell line (AB266272)
Representative images of JUP knockout HEK293T cells, low and high confluency examples (top left and right respectively) and wild-type HEK293T cells, low and high confluency (bottom left and right respectively) showing typical adherent, epithelial-like morphology. Images were captured at 10X magnification using a EVOS XL Core microscope.
- Sanger seq
Unknown
Sanger Sequencing - Human JUP (gamma Catenin) knockout HEK-293T cell line (AB266272)
Homozygous : 1 bp deletion in exon 2
Reactivity data
Product details
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Gamma Catenin is an integral component of the armadillo family of proteins and part of desmosomal and adherens junction complexes. It plays an essential role in maintaining the integrity and mechanical strength of tissues. Through interacting with other proteins in these complexes gamma Catenin facilitates cell signaling and communication. It associates with cadherins and catenins including plakophilin and β-catenin to ensure proper cell-cell adhesion and signaling across the cell membrane.
Pathways
Gamma Catenin is involved in the Wnt signaling pathway an important route governing cellular processes such as proliferation differentiation and migration. Gamma Catenin interacts with β-catenin in this pathway influencing transcriptional regulation in the nucleus. It also plays roles in the cadherin-mediated cell adhesion pathway working alongside other proteins like E-cadherin to modulate cell behavior. These pathways highlight its importance in cellular architecture and signaling.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Target data
Complementary Products
Primary Antibodies
AB184919
Anti-gamma Catenin antibody [EPR17310]
RabMAb|Recombinant|KO Validated
![Immunocytochemistry/ Immunofluorescence - Anti-gamma Catenin antibody [EPR17310] (AB184919)](https://content.abcam.com/products/images/gamma-catenin-antibody-epr17310-ab184919--immunocytochemistry-immunofluorescence-img68699.jpg)
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Cell Lines & Lysates
AB266343
Human RAD23A (hHR23A) knockout HEK-293T cell line
Advanced Validation
- 0
- 0
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com