Human KAT2A knockout A549 cell line

• WB

Lab

Western blot - Human KAT2A knockout A549 cell line (AB287576)

Western blot : Anti-KAT2A antibody [EPR21146] (ab217876) staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab217876 was shown to bind specifically to KAT2A. A band was observed at 95 kDa in wild-type A549 cell lysates with no signal observed at this size in KAT2A knockout cell line. To generate this image, wild-type and KAT2A knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-KAT2A / GCN5 antibody [EPR21146] - ChIP Grade (<a href='/en-us/products/primary-antibodies/kat2a-gcn5-antibody-epr21146-chip-grade-ab217876'>ab217876</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 cell lysate at 20 µg

Lane 2:

KAT2A knockout A549 cell lysate at 20 µg

Lane 3:

Wild-type U-2 OS cell lysate at 20 µg

Lane 4:

GCN5 knockout U-2 OS cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

false

• WB

Lab

Western blot - Human KAT2A knockout A549 cell line (AB287576)

Western blot : Anti-KAT2A antibody [AT3G13] (ab208097) staining at 1/1000 dilution, shown in green; Rabbit anti-alpha Tubulin antibody [EP1332Y] (ab52866) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab208097 was shown to bind specifically to KAT2A. A band was observed at 95 kDa in wild-type A549 cell lysates with no signal observed at this size in KAT2A knockout cell line. To generate this image, wild-type and KAT2A knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L 800CW and Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-KAT2A / GCN5 antibody [AT3G13] (<a href='/en-us/products/primary-antibodies/kat2a-gcn5-antibody-at3g13-ab208097'>ab208097</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 cell lysate at 20 µg

Lane 2:

KAT2A knockout A549 cell lysate at 20 µg

Lane 3:

Wild-type U-2 OS cell lysate at 20 µg

Lane 4:

GCN5 knockout U-2 OS cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Mouse IgG H&L 800CW and Goat anti-Rabbit IgG H&L 680RD at 1/20000 dilution

false

• NGS

Lab

Next Generation Sequencing - Human KAT2A knockout A549 cell line (AB287576)

1 bp insertion after Ser 243 of WT protein, and 78 bp deletion (Allele 1); 142 bp deletion after Ser 243 of WT protein (Allele 2); 53 bp insertion after Ser 243 of wt protein, 79 bp deletion downstream