KDELR2 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1.
Images
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1
Alternative names
(Lys Asp Glu Leu) endoplasmic reticulum protein retention receptor 2, ER lumen protein retaining receptor 2, ERD 2 like protein, ERD2-like protein 1, ERD2.2, ERD22_HUMAN, FLJ45532, KDEL (Lys Asp Glu Leu) endoplasmic reticulum protein retention receptor 2, KDEL endoplasmic reticulum protein retention receptor 2, KDEL receptor 2, KDELR 2
Recommended products
KDELR2 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1.
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1
- Concentration
Properties
- Gene name
- KDELR2
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
- Zygosity
- Homozygous
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ELP-1 also known as Elongator complex protein 1 or KDEL receptor 2 is a protein with a molecular weight of approximately 150 kDa. This protein is an important component of the Elongator complex which is involved in transcriptional elongation by RNA polymerase II. ELP-1 is ubiquitously expressed in various tissues suggesting its significance across multiple cell types. Its presence within the Elongator complex a multi-subunit assembly facilitates the proper modification of tRNA impacting protein synthesis efficiency.
- Biological function summary
ELP-1 plays an important role in facilitating the modification of tRNA wobble uridines. This allows for accurate and efficient translation of the genetic code into proteins. ELP-1 as a vital part of the Elongator complex also contributes to the acetylation of histones which impacts chromatin structure and gene expression regulation. The protein ensures that gene transcription proceeds effectively by interacting with different components of the transcription machinery.
- Pathways
The Elongator complex including ELP-1 integrates into the transcriptional regulation and tRNA modification pathways. ELP-1 interacts with several proteins like RNA polymerase II during gene transcription. Another intersecting pathway includes the regulation of response to cellular stress as correct modification of tRNA maintains protein synthesis under stress conditions. Its functionality within these pathways illustrates its integral role in ensuring proper cellular response to environmental changes.
- Associated diseases and disorders
ELP-1 is linked to familial dysautonomia a neurodevelopmental disorder that affects autonomic nervous system functioning. Mutations in the IKBKAP gene encoding ELP-1 impair its function leading to the disease's manifestation. Additionally there is evidence suggesting ELP-1's involvement in multiple cancer types where altered elongator complex activity can affect tumor progression. In these contexts ELP-1's interactions with other proteins like IKBKAP become highly relevant for understanding the disease mechanisms and potentially developing therapeutic interventions.
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2 product images
Sanger Sequencing - Human KDELR2 (ELP-1) knockout HEK-293T cell line (ab266511)
Homozygous: 1 bp insertion in exon 1
Sanger Sequencing - Human KDELR2 (ELP-1) knockout HEK-293T cell line (ab266511)
Sequencing chromatogram displaying sequence edit in exon 1
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