Human KDM1A (LSD1) knockout HeLa cell line
- Advanced Validation
- What is this?
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KDM1A KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 4 bp deletion in exon 1. To order both knockout and wild-type control cells: select '2 x 1000000 Cells/vial'. To order only knockout cells: select '1000000 Cells/vial'.
View Alternative Names
AOF2, Amine oxidase (flavin containing) domain 2, Amine oxidase, flavin containing, 2, BHC110, BRAF35-HDAC complex protein BHC110, BRAF35/HDAC complex, 110-kD subunit, CPRF, EC1, FAD-binding protein BRAF35-HDAC complex, 110 kDa subunit, Flavin-containing amine oxidase domain-containing protein 2, KDM 1, KDM1A_HUMAN, KIAA0601, LSD 1, Lysine (K) specific demethylase 1, Lysine (K) specific demethylase 1A, Lysine demethylase 1A, Lysine-specific demethylase 1, Lysine-specific demethylase 1A, Lysine-specific histone demethylase 1, Lysine-specific histone demethylase 1A
- WB
Lab
Western blot - Human KDM1A (LSD1) knockout HeLa cell line (AB265790)
Lanes 1- 2 : Merged signal (red and green). Green - ab129195 observed at 110 kDa. Red - loading control ab8245 observed at 37 kDa.
ab129195 Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker was shown to specifically react with KDM1/LSD1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265790 (knockout cell lysate ab256965) was used. Wild-type and KDM1/LSD1 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab129195 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-KDM1/LSD1 antibody [EPR6825] - Nuclear Marker and ChIP Grade (<a href='/en-us/products/primary-antibodies/kdm1-lsd1-antibody-epr6825-nuclear-marker-and-chip-grade-ab129195'>ab129195</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
KDM1A knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human KDM1A (LSD1) knockout HeLa cell line (ab265790)
Predicted band size: 155 kDa,92 kDa
Observed band size: 110 kDa,160 kDa
false
- WB
Lab
Western blot - Human KDM1A (LSD1) knockout HeLa cell line (AB265790)
Lanes 1- 2 : Merged signal (red and green). Green - ab69535 observed at 110 kDa. Red - loading control ab181602 observed at 37 kDa.
ab69535 Anti-KDM1/LSD1 antibody [1B2F2] was shown to specifically react with KDM1/LSD1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265790 (knockout cell lysate ab256965) was used. Wild-type and KDM1/LSD1 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab69535 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-KDM1/LSD1 antibody [1B2F2] (<a href='/en-us/products/primary-antibodies/kdm1-lsd1-antibody-1b2f2-ab69535'>ab69535</a>)
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
KDM1 knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human KDM1A (LSD1) knockout HeLa cell line (ab265790)
Predicted band size: 92 kDa
Observed band size: 110 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human KDM1A (LSD1) knockout HeLa cell line (AB265790)
Homozygous : 4 bp deletion in exon 1.
Reactivity data
Product details
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Target data
Complementary Products
Primary Antibodies
AB17721
Anti-KDM1/LSD1 antibody - Nuclear Marker
KO Validated|Lab Essentials
- 4
- 25
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com