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KDM1B KO cell line available to order. Free of charge wild type control provided.

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Key facts

Cell type

PC-3

Species or organism

Human

Tissue

Prostate

Form

Liquid

Alternative names

Recommended products

KDM1B KO cell line available to order. Free of charge wild type control provided.

Key facts

Cell type

PC-3

Form

Liquid

Disease

Adenocarcinoma

Concentration
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Properties

Gene name

KDM1B

Gene editing type

Knockout

Gene editing method

CRISPR technology

Cell culture

Biosafety level

EU: 1 US: 1

Adherent/suspension

Adherent

Gender

Male

Handling procedures

Initial handling guidelines

Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.

1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.

Subculture guidelines

  • All seeding densities should be based on cell counts gained by established methods.

  • A guide seeding density of 2x104 cells/cm2 is recommended.

  • Cells should be passaged when they have achieved 80-90% confluence.

Culture medium

F-12K + 10% FBS

Cryopreservation medium

Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.

Storage

Shipped at conditions

Dry Ice

Appropriate short-term storage conditions

-196°C

Appropriate long-term storage conditions

-196°C

Notes

Although we aim to provide customers with a homozygous clone, feasibility will be dependent on the biology of the protein. Should only heterozygous edits be achieved, you will be notified of the outcome and be asked to confirm whether the cell line is acceptable. All clones will be accompanied with DNA sequencing data, and the mutation description.

Recommended control: Human wild-type PC-3 cell line (Human wild-type PC-3 cell line ab290718). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.

We will provide viable cells that proliferate on revival.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

LSD2 also known as AOF1 is a lysine-specific demethylase enzyme with a mass of about 105 kDa. This protein mainly functions by removing methyl groups from lysine residues on histone proteins thereby affecting chromatin structure and gene expression. LSD2 is expressed in various tissues with high levels in the liver and brain. The enzyme plays a significant role in regulating gene activity influencing many biological processes.

Biological function summary

LSD2 impacts gene expression by altering the epigenetic state of chromatin. It is not part of a well-defined large multiprotein complex allowing LSD2 to directly interact with histones. Through its demethylase activity it modulates the transcriptional regulation impacting cellular differentiation and proliferation. This makes it an important factor in maintaining cellular homeostasis.

Pathways

LSD2 is involved in histone modification processes particularly through the demethylation of H3K4me1 and H3K4me2 marks. These changes are important in the regulation of gene expression patterns. LSD2 often interacts with the REST corepressor complex affecting the expression of genes involved in neural development and function. It plays a role in pathways related to cellular stress responses and metabolic regulation highlighting its importance in cellular adaptation mechanisms.

Associated diseases and disorders

LSD2 has been implicated in cancer and neurological disorders. Alterations in its expression and activity are associated with certain types of cancers such as breast cancer where it affects tumor growth and progression by influencing gene expression patterns. In neurological contexts LSD2's dysregulation impacts neural circuitry and cognitive functions which can be linked to disorders like Alzheimer's disease. In these conditions LSD2 interacts with proteins such as SIRT1 influencing cellular processes that contribute to disease pathology.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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    For this product, it's our understanding that no specific protocols are required. You can:

    Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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