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AB266252

Human KDM5C (Jarid1C / SMCX) knockout HEK-293T cell line

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KDM5C KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 17 bp deletion in exon 4 and 5 bp deletion in exon 4. To order both knockout and wild-type control cells: select '2 x 1000000 Cells/vial'. To order only knockout cells: select '1000000 Cells/vial'.
4 Images
Western blot - Human KDM5C (Jarid1C / SMCX) knockout HEK-293T cell line (AB266252)
  • WB

Lab

Western blot - Human KDM5C (Jarid1C / SMCX) knockout HEK-293T cell line (AB266252)

Blocking and Diluting buffer and concentration : 5% NFDM/TBST

Lanes 1-4 : Merged signal (red and green). Green - ab259913 observed at 180 kDa. Red - loading control ab8245 (Mouse monoclonal [6C5] to GAPDH) observed at 36 kDa.

ab259913 Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] was shown to specifically react with KDM5C / Jarid1C / SMCX in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266251 (knockout cell lysate ab257494) and ab266252 (knockout cell lysate ab257495) were used. Wild-type and KDM5C / Jarid1C / SMCX knockout samples were subjected to SDS-PAGE. ab259913 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at 4℃ overnight at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] (<a href='/en-us/products/primary-antibodies/kdm5c-jarid1c-smcx-antibody-epr23932-18-ab259913'>ab259913</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK293T (human embryonic kidney epithelial cell), whole cell lysate at 20 µg

Lane 2:

Western blot - Human KDM5C (Jarid1C / SMCX) knockout HEK-293T cell line (ab266252) at 20 µg

Lane 3:

Western blot - Human KDM5C (Jarid1C / SMCX) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-kdm5c-jarid1c-smcx-knockout-hek-293t-cell-line-ab266251'>ab266251</a>) at 20 µg

Lane 4:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (IRDye® 800CW) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) and Goat Anti-Mouse IgG H&L (IRDye® 680RD) (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-680rd-preadsorbed-ab216776'>ab216776</a>)

Predicted band size: 175 kDa

Observed band size: 180 kDa

false

Sanger Sequencing - Human KDM5C (Jarid1C / SMCX) knockout HEK-293T cell line (AB266252)
  • Sanger seq

Unknown

Sanger Sequencing - Human KDM5C (Jarid1C / SMCX) knockout HEK-293T cell line (AB266252)

Allele-1 : 17 bp deletion in exon4

Sanger Sequencing - Human KDM5C (Jarid1C / SMCX) knockout HEK-293T cell line (AB266252)
  • Sanger seq

Unknown

Sanger Sequencing - Human KDM5C (Jarid1C / SMCX) knockout HEK-293T cell line (AB266252)

Allele-2 : 5 bp deletion in exon 4.

Western blot - Human KDM5C (Jarid1C / SMCX) knockout HEK-293T cell line (AB266252)
  • WB

Supplier Data

Western blot - Human KDM5C (Jarid1C / SMCX) knockout HEK-293T cell line (AB266252)

This data was developed using ab259913, the same antibody clone in a different buffer formulation.

Blocking and Diluting buffer and concentration : 5% NFDM/TBST

Lanes 1-4 : Merged signal (red and green). Green - ab259913 observed at 180 kDa. Red - loading control ab8245 (Mouse monoclonal [6C5] to GAPDH) observed at 36 kDa.

Lanes 1-3 : ab259913 Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] was shown to specifically react with KDM5C / Jarid1C / SMCX in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266251 (knockout cell lysate ab257494) and ab266252 (knockout cell lysate ab257495) were used. Wild-type and KDM5C / Jarid1C / SMCX knockout samples were subjected to SDS-PAGE. ab259913 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at 4℃ overnight at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-KDM5C / Jarid1C / SMCX antibody [EPR23932-18] (<a href='/en-us/products/primary-antibodies/kdm5c-jarid1c-smcx-antibody-epr23932-18-ab259913'>ab259913</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK293T (human embryonic kidney epithelial cell), whole cell lysate at 20 µg

Lane 2:

Western blot - Human KDM5C (Jarid1C / SMCX) knockout HEK-293T cell line (ab266252) at 20 µg

Lane 3:

Western blot - Human KDM5C (Jarid1C / SMCX) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-kdm5c-jarid1c-smcx-knockout-hek-293t-cell-line-ab266251'>ab266251</a>) at 20 µg

Lane 4:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (IRDye® 800CW) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) and Goat Anti-Mouse IgG H&L (IRDye® 680RD) (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-680rd-preadsorbed-ab216776'>ab216776</a>) at 1/10000 dilution

Predicted band size: 175 kDa

Observed band size: 180 kDa

false

Key facts

Cell type

HEK-293T

Species or organism

Human

Tissue

Kidney

Form

Liquid

form

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, 17 bp deletion in exon 4 and 5 bp deletion in exon 4

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Complementary Products

Primary Antibodies

AB34718

Anti-KDM5C / Jarid1C / SMCX antibody

KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-KDM5C / Jarid1C / SMCX antibody (AB34718)

  • 4
  • 5
Primary Antibodies

AB108592

Anti-hHR23A antibody [EPR4818]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-hHR23A antibody [EPR4818] (AB108592)

  • 5
  • 3
Primary Antibodies

AB171933

Anti-VRK1 antibody [5D1] - N-terminal

KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-VRK1 antibody [5D1] - N-terminal (AB171933)

  • 0
  • 0
Primary Antibodies

AB109524

Anti-Topoisomerase II alpha + Topoisomerase II beta/TOP2B antibody [EPR5377]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Topoisomerase II alpha + Topoisomerase II beta/TOP2B antibody [EPR5377] (AB109524)

  • 5
  • 2
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Reactivity data

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Product details

We will provide viable cells that proliferate on revival.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
KDM5C
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-196°C
Appropriate long-term storage conditions
-196°C
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Handling procedures

Initial handling guidelines

Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.

1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.

Subculture guidelines
  • All seeding densities should be based on cell counts gained by established methods.
  • A guide seeding density of 2x104 cells/cm2 is recommended.
  • Cells should be passaged when they have achieved 80-90% confluence.
Culture medium

DMEM (High Glucose) + 10% FBS

Cryopreservation medium

Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.

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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Target data

See full target information KDM5C
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com