Skip to main content

KRT1 KO cell line available to order. Free of charge wild type control provided.

Be the first to review this product! Submit a review

Key facts

Cell type
HCT116
Species or organism
Human
Tissue
Colon
Form
Liquid

Alternative names

Recommended products

KRT1 KO cell line available to order. Free of charge wild type control provided.

Key facts

Cell type
HCT116
Form
Liquid
Disease
Carcinoma
Concentration
Loading...

Properties

Gene name
KRT1
Gene editing type
Knockout
Gene editing method
CRISPR technology

Quality control

STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

Cell culture

Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male

Handling procedures

Initial handling guidelines

Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.

1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.

Subculture guidelines
  • All seeding densities should be based on cell counts gained by established methods.
  • A guide seeding density of 2x104 cells/cm2 is recommended.
  • Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
McCoY5a + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.

Storage

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-196°C
Appropriate long-term storage conditions
-196°C

Notes

Although we aim to provide customers with a homozygous clone, feasibility will be dependent on the biology of the protein. Should only heterozygous edits be achieved, you will be notified of the outcome and be asked to confirm whether the cell line is acceptable. All clones will be accompanied with DNA sequencing data, and the mutation description.

Recommended control: Human wild-type HCT116 cell line (ab288559). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.

We will provide viable cells that proliferate on revival.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Cytokeratins also known by the abbreviation 'CK' are a class of intermediate filament proteins providing structural support to epithelial cells. Their mass ranges between 40 kDa to 68 kDa. Cytokeratins are expressed in epithelial tissues including the outer layer of skin and line organs such as the gut and respiratory tract. Commonly referenced with specific subtypes CK MNF116 or CK cocktail antibodies are used for detection in laboratory settings. These proteins help maintain cell integrity and protect from mechanical stress.

Biological function summary

Keratins function as a complex to create a stable cytoskeletal network inside epithelial cells. They interact with proteins to form keratin filaments contributing to the mechanical strength and resilience of cells. Cytokeratins also play a role in intracellular signaling and cellular differentiation. They participate in wound healing processes through modulation of cell proliferation and apoptosis. The CK MNF116 marker is especially noted for its application in identifying and characterizing epithelial proteins in pathology.

Pathways

Cytokeratins are involved in pathways related to cell structure and stress response. Specifically they play key roles in the Epidermal Growth Factor (EGF) signaling pathway and the cell cycle control pathway. These pathways involve proteins like epidermal growth factor receptors (EGFR) and cyclins which regulate cell cycle progression. Cytokeratins influence these pathways by impacting cell adhesion and proliferation essential for maintaining epithelial cell functions.

Associated diseases and disorders

Cytokeratins have significant roles in cancer diagnostics particularly in epithelial tumors such as carcinomas. Altered expression of cytokeratins can indicate the presence and progression of tumors. Cytokeratin profiles including CK MNF116 positive markers provide insights into tumor type and origin. Additionally they relate to liver disorders like cirrhosis where abnormal keratin organization is observed. These proteins show associations with other structural proteins including vimentin in disease states where cell integrity and signaling are often compromised.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

0 product images

    Downloads

    Product protocols

    For this product, it's our understanding that no specific protocols are required. You can:

    Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

    For licensing inquiries, please contact partnerships@abcam.com