L1CAM KO cell line available to order. KO validated by Next Generation Sequencing. Free of charge wild type control provided. Knockout achieved by CRISPR/Cas9 X = 1 bp insertion Frameshift: 96%.
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Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Next Generation Sequencing
- Mutation description
- Knockout achieved by CRISPR/Cas9 X = 1 bp insertion Frameshift: 96%
Alternative names
Antigen identified by monoclonal antibody R1, CAML1, CD171, CD171 antigen, HSAS, HSAS1, Hyd, L1, L1 cell adhesion molecule, L1-NCAM, L1CAM_HUMAN, MIC5, N-CAM-L1, NILE, Nerve-growth factor-inducible large external glycoprotein, Neural cell adhesion molecule L1, OTTHUMP00000025992
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L1CAM KO cell line available to order. KO validated by Next Generation Sequencing. Free of charge wild type control provided. Knockout achieved by CRISPR/Cas9 X = 1 bp insertion Frameshift: 96%.
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Form
- Liquid
- Knockout validation
- Next Generation Sequencing
- Mutation description
- Knockout achieved by CRISPR/Cas9 X = 1 bp insertion Frameshift: 96%
- Disease
- Adenocarcinoma
- Concentration
Properties
- Gene name
- L1CAM
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Next Generation Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
L1CAM also known as neural cell adhesion molecule L1 is a transmembrane protein with a molecular weight of approximately 200-220 kDa. The L1CAM protein belongs to the immunoglobulin superfamily and is heavily glycosylated. It plays a significant role in cell adhesion processes within the nervous system. L1CAM is expressed in a variety of tissues including the brain kidney and peripheral nerves and is notably present in neuronal cells where it engages in cell-cell interactions. Researchers frequently use L1CAM IHC ELISA methods and specific fluorescent markers like Alexa Fluor 568 to study its expression patterns and localization.
- Biological function summary
L1CAM facilitates neuronal migration axonal growth and synaptic plasticity. This protein participates in the formation and maintenance of neural networks. It interacts with other cell adhesion molecules and components of the extracellular matrix. L1CAM acts as an important modulator within neuronal signaling complexes which impacts the development and regeneration of the nervous system. Insights into its structural and cell interaction properties have made it a focus for understanding nervous system functions.
- Pathways
L1CAM participates extensively in the MAPK and PI3K/AKT pathways. These pathways contribute to cellular growth survival and programmed cell death. The protein interacts with other molecules such as integrins and FGFRs to propagate signaling cascades important for cellular response mechanisms. These connections help modulate cytoskeletal dynamics influencing cellular adhesion and motility needed during brain development and response to injury.
- Associated diseases and disorders
L1CAM has been associated with hydrocephalus and some cancers. Mutations in the L1CAM gene can lead to L1 syndrome which includes a spectrum of neurological disorders particularly hydrocephalus. In cancer overexpression or altered regulation of L1CAM may correlate with tumor aggressiveness and metastatic potential. L1CAM's association with integrin and cadherin proteins underlines its relevance in pathological states and highlights a potential target for therapeutic interventions.
Product promise
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2 product images
Next Generation Sequencing - Human L1CAM knockout HeLa cell line (ab273836)
Knockout achieved by CRISPR/Cas9; X = 1 bp insertion; Frameshift: 96%
Next Generation Sequencing - Human L1CAM knockout HeLa cell line (ab273836)
1 bp deletion after Val35 of the WT protein
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