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LAMTOR1 KO cell line available to order. KO validated by Western blot. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and 4 bp insertion in exon 1.

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Images

Western blot - Human LAMTOR1 knockout HeLa cell line (AB265351), expandable thumbnail
  • Sanger Sequencing - Human LAMTOR1 knockout HeLa cell line (AB265351), expandable thumbnail
  • Sanger Sequencing - Human LAMTOR1 knockout HeLa cell line (AB265351), expandable thumbnail

Key facts

Cell type
HeLa
Species or organism
Human
Tissue
Cervix
Form
Liquid
Knockout validation
Sanger Sequencing, Western blot
Mutation description
Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and 4 bp insertion in exon 1

Alternative names

C11orf59, LTOR1_HUMAN, Late endosomal/lysosomal adaptor and MAPK and MTOR activator 1, Lipid raft adaptor protein p18, PDRO, PP7157, Protein associated with DRMs and endosomes, Ragulator complex protein LAMTOR1, Ragulator1, RhoA activator C11orf59, p18, p27Kip1-releasing factor from RhoA, p27RF-Rho, ragulator complex protein PDRO

Recommended products

LAMTOR1 KO cell line available to order. KO validated by Western blot. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and 4 bp insertion in exon 1.

Key facts

Cell type
HeLa
Form
Liquid
Mutation description
Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and 4 bp insertion in exon 1
Disease
Adenocarcinoma
Concentration
Loading...

Properties

Gene name
LAMTOR1
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot

Quality control

STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

Cell culture

Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female

Handling procedures

Initial handling guidelines

Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.

1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.

Subculture guidelines
  • All seeding densities should be based on cell counts gained by established methods.
  • A guide seeding density of 2x104 cells/cm2 is recommended.
  • Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.

Storage

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-196°C
Appropriate long-term storage conditions
-196°C

Notes

Recommended control: Human wild-type HeLa cell line (ab255928). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.

We will provide viable cells that proliferate on revival.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

LAMTOR1 also known as p18 is a protein with a mass of approximately 18 kDa. It functions mechanically as a scaffold protein located on the late endosome/lysosome membrane. The protein's primary role involves recruiting and stabilizing the other members of the Ragulator complex. LAMTOR1 is expressed in various tissues with significant levels in immune and neuronal cells where it supports cellular growth and immune responses.

Biological function summary

LAMTOR1 acts as a part of the highly-conserved Ragulator complex. The Ragulator complex is vital for the activation of the mechanistic target of rapamycin complex 1 (mTORC1) in response to amino acids. LAMTOR1's interaction with other components of the complex such as LAMTOR2 and LAMTOR3 facilitates the localization and activation of mTORC1 on the lysosomal surface. This activation promotes anabolic processes including protein lipid and nucleotide synthesis.

Pathways

LAMTOR1 plays an important role in the mTOR signaling pathway. This pathway regulates cell growth proliferation and metabolism. LAMTOR1's involvement with Rag proteins assists in sensing amino acid levels thereby influencing the mTORC1 pathway activation. Additionally LAMTOR1-related pathways intersect with the AMPK signaling pathway where it functionally interacts with proteins that control cellular energy homeostasis.

Associated diseases and disorders

LAMTOR1's dysregulation has been associated with conditions like cancer and neurodegenerative disorders. Altered expression of LAMTOR1 can impact mTORC1 signaling leading to uncontrolled cell growth often observed in cancerous cells. In neurodegenerative disorders defects in mTORC1 pathway can cause neuronal cell damage. These conditions link LAMTOR1 to proteins like Rheb and TSC2 which are important for maintaining a balance in these signaling pathways.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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