Human LATS1 (WARTS) knockout THP-1 cell line
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- WB
Lab
Western blot - Human LATS1 (WARTS) knockout THP-1 cell line (AB277862)
Western blot : Anti-LATS1 antibody [EPR23057-116] (ab243656) staining at 1/2000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab243656 was shown to bind specifically to LATS1. A band was observed at 127 kDa in wild-type THP-1 cell lysates with no signal observed at this size in LATS1 knockout cell line. To generate this image, wild-type and LATS1 knockout THP-1 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes:
Western blot - Anti-LATS1/WARTS antibody [EPR23057-116] (<a href='/en-us/products/primary-antibodies/lats1-warts-antibody-epr23057-116-ab243656'>ab243656</a>) at 1/2000 dilution
Lane 1:
Wild-type THP-1 cell lysate at 20 µg
Lane 2:
Western blot - Human LATS1 (WARTS) knockout THP-1 cell line (ab277862)
Lane 2:
LATS1 knockout THP-1 cell lysate at 20 µg
Lane 3:
HT-29 cell lysate at 20 µg
Lane 4:
PC-3 cell lysate at 20 µg
Lane 5:
HeLa cell lysate at 20 µg
Secondary
All lanes:
Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Predicted band size: 126 kDa
Observed band size: 127 kDa
false
- WB
Lab
Western blot - Human LATS1 (WARTS) knockout THP-1 cell line (AB277862)
Anti-LATS1 antibody [C66B5] staining at 1/1000 dilution, shown in black; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab3477S was shown to bind specifically to LATS1. A band was observed at 100-150 kDa in wild-type THP-1 cell lysates with no signal observed at this size in LATS1 knockout cell line. To generate this image, wild-type and LATS1 knockout THP-1 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % BSA in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times before development with a high-sensitivity ECL substrate kit and imaged with 20 minutes exposure time. Secondary antibodies used were HRP conjugated Goat anti-Rabbit (H+L) and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes:
Anti-LATS1 antibody at 1/1000 dilution
Lane 1:
Wild-type THP-1 cell lysate at 20 µg
Lane 2:
Western blot - Human LATS1 (WARTS) knockout THP-1 cell line (ab277862)
Lane 2:
LATS1 knockout THP-1 cell lysate at 20 µg
Lane 3:
HT-29 cell lysate at 20 µg
Lane 4:
PC-3 cell lysate at 20 µg
Lane 5:
HeLa cell lysate at 20 µg
Lane 6:
NIH/3T3 cell lysate at 20 µg
Predicted band size: 126 kDa
false
- Sanger seq
Supplier Data
Sanger Sequencing - Human LATS1 (WARTS) knockout THP-1 cell line (AB277862)
31 bp deletion in exon 3.
Complementary Products
Product details
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
LATS1/WARTS functions as an important regulator of cellular growth and differentiation. It is part of the Hippo signaling complex which includes proteins like MST1/2 and YAP/TAZ. This complex helps maintain proper tissue homeostasis by modulating gene expression in response to cellular density and mechanical cues. In doing so LATS1/WARTS phosphorylates and inactivates downstream effectors YAP and TAZ preventing them from translocating to the nucleus where they could promote transcription of growth-promoting genes.
Pathways
Scientists have found LATS1/WARTS to be integral to both the Hippo signaling pathway and the cell cycle regulation pathway. Within these pathways LATS1/WARTS interacts closely with proteins such as MOB1 and YAP mediating cellular responses to growth inhibitory signals. By controlling these interactions LATS1/WARTS helps coordinate cell proliferation and apoptosis which is critical for proper developmental processes and prevention of tumor growth.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Suspension
Gender
Male
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability. <p>1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.<br>2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.<br>3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method. Based on cell count, seed cells in an appropriate cell culture flask at a density of 4x10<sup>5</sup> cells/mL. Seeding density is given as a guide only and should be scaled to align with individual lab schedules.<br>4. Incubate the culture at 37°C incubator with 5% CO<sub>2</sub>. Cultures should be monitored daily.<br>5. THP-1 cells recover slowly from cryopreservation and therefore may not be ready for subculture for a number of days. Cells should be left as much as possible over this time and only subcultured when the cell density reaches 8x10<sup>5</sup> cells/mL.Small amounts of fresh media can be added until cell number/viability improves.</p>
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- Cells should be seeded at 2x105 - 3x105 cells/mL and subcultured when they have reached 8x105 cells/mL.
- It is not recommended to allow the cell density to exceed 1x106 cells/mL.
Culture medium
RPMI + 10% FBS + 0.05 mM beta-mercaptoethanol
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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