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LMOD1 KO cell line available to order. Free of charge wild type control provided.

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Sanger Sequencing - Human LMOD1 knockout SH-SY5Y cell line (AB282566), expandable thumbnail

Key facts

Cell type

SH-SY5Y

Species or organism

Human

Tissue

Bone marrow

Form

Liquid

Knockout validation

Sanger Sequencing

Alternative names

Recommended products

LMOD1 KO cell line available to order. Free of charge wild type control provided.

Key facts

Cell type

SH-SY5Y

Form

Liquid

Disease

Neuroblastoma

Concentration
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Properties

Gene name

LMOD1

Gene editing type

Knockout

Gene editing method

CRISPR technology

Knockout validation

Sanger Sequencing

Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

Cell culture

Biosafety level

EU: 1 US: 1

Adherent/suspension

Adherent

Gender

Female

Handling procedures

Initial handling guidelines

Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.

1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.

Subculture guidelines

  • All seeding densities should be based on cell counts gained by established methods.

  • These cells grow as a mixture of floating and adherent cells.

  • Remove media containing floating cells and recover cells by centrifugation, detach cells using standard methods, combine with floating cells and transfer to a new culture flask.

  • A guide seeding density of 2x104 cells/cm2 is recommended.

  • Cells should be seeded at a density conducive to cell–cell communication to proliferate. If cells are seeded too sparsely, growth rate is reduced and cell death is high.

Culture medium

1:1 mixture of EMEM and F-12K + 10% FBS

Cryopreservation medium

Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.

Storage

Shipped at conditions

Dry Ice

Appropriate short-term storage conditions

-196°C

Appropriate long-term storage conditions

-196°C

Notes

Recommended control: Human wild-type SHSY-5Y cell line (Human wild-type SH-SY5Y cell line ab275475). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.

We will provide viable cells that proliferate on revival.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

LMOD1 also known as Leiomodin 1 is a protein involved in actin filament dynamics. It has a molecular mass of approximately 64 kDa. The protein plays a role in the regulation of actin nucleation and assembly which are critical for the structural integrity and function of muscle cells. LMOD1 is expressed in smooth muscle tissues including those in the vascular system. This expression highlights its functional significance in maintaining smooth muscle contractility.

Biological function summary

LMOD1 contributes to muscle cell contraction by stabilizing and organizing actin filaments. It functions by binding to the pointed ends of actin filaments and may form a part of a multiprotein complex with actin and other actin-binding proteins. This association enhances its capability to regulate actin filament growth and stability factors essential for maintaining cellular structure and motility in smooth muscle tissues. LMOD1's ability to influence actin filament dynamics highlights its significance in muscle cell function and integrity.

Pathways

LMOD1 impacts actin dynamics critical to the smooth muscle contraction pathway. It interacts with proteins such as tropomyosin and tropomodulin influencing actin filament stabilization and organization. The smooth muscle contraction pathway is essential for vascular tone and blood flow regulation. LMOD1's association with actin regulation aligns its role within this pathway impacting smooth muscle cellular contractility and overall smooth muscle function.

Associated diseases and disorders

LMOD1 is associated with vascular diseases such as arteriosclerosis due to its role in smooth muscle function. Alterations in LMOD1 expression or mutations can lead to abnormal muscle contraction and vascular issues. Additionally LMOD1 has links to hypertensive conditions where dysregulated smooth muscle contraction affects blood pressure control. In these conditions LMOD1 interacts with proteins like actin and tropomyosin which play a role in cellular contractility and are critical in maintaining normal vascular function.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Sanger Sequencing - Human LMOD1 knockout SH-SY5Y cell line (ab282566), expandable thumbnail

    Sanger Sequencing - Human LMOD1 knockout SH-SY5Y cell line (ab282566)

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com