Human LYPLA2 (APT-2) knockout HEK-293T cell line
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LYPLA2 KO cell line available to order. KO validated by. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 7 bp deletion in exon 2.
View Alternative Names
APT-2, Acyl protein thioesterase, Acyl-protein thioesterase 2, DJ886K2.4, EC 3.1.2.-, LPL-I, LPL-II, LYPA2_HUMAN, LYPLA 2, LysoPLA II, Lysophospholipase II, OTTHUMP00000002999, OTTHUMP00000044831
- Sanger seq
Unknown
Sanger Sequencing - Human LYPLA2 (APT-2) knockout HEK-293T cell line (AB266696)
Homozygous : 7 bp deletion in exon2
Product details
Recommended control: Human wild-type HEK293T cell line (ab255449). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
APT-2 plays a critical role in maintaining phospholipid homeostasis contributing to membrane fluidity and signal transduction. It is involved in hydrolyzing lysophospholipids into lysoglycerophospholipids and free fatty acids. Not part of a complex APT-2 acts as an individual enzyme handling specific substrates in this context. Its activity regulates levels of lipid signaling molecules influencing cellular responses to environmental cues. APT-2 also contributes to energy storage via metabolism of lipid substrates linking its enzymatic activity to broader metabolic pathways.
Pathways
APT-2 holds significance in both metabolic and signaling pathways specifically the glycerophospholipid metabolism and lysophospholipid signaling pathways. It catalyzes reactions key to these pathways and intersects with proteins like phospholipase A2 and PLA2G6. Glycerophospholipid metabolism sees APT-2 breaking down molecules to sustain membrane integrity and cellular communication processes. In lysophospholipid signaling its activity regulates bioactive lipid levels which impact a variety of cellular signaling events showing a well-integrated role in cellular physiology.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Target data
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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