MAPK14 KO cell line available to order. Free of charge wild type control provided.
CSAID Binding Protein 1, CSAID-binding protein, CSBP, CSBP 1, CSBP 2, CSPB1, Csaids binding protein, Cytokine suppressive anti-inflammatory drug-binding protein, EXIP, MAP kinase 14, MAP kinase MXI2, MAP kinase p38 alpha, MAPK 14, MAX-interacting protein 2, MK14_HUMAN, Mitogen-activated protein kinase 14, Mitogen-activated protein kinase p38 alpha, Mxi 2, P38, PRKM14, PRKM15, RK, SAPK2A, Stress-activated protein kinase 2a, p38 ALPHA, p38 MAP kinase, p38 MAPK, p38 mitogen activated protein kinase, p38alpha Exip
MAPK14 KO cell line available to order. Free of charge wild type control provided.
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Although we aim to provide customers with a homozygous clone, feasibility will be dependent on the biology of the protein. Should only heterozygous edits be achieved, you will be notified of the outcome and be asked to confirm whether the cell line is acceptable. All clones will be accompanied with DNA sequencing data, and the mutation description.
Recommended control: Human wild-type A549 cell line (Human wild-type A549 cell line ab288558). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
P38 also known as MAPK14 is a member of the mitogen-activated protein kinase (MAPK) family. It functions by phosphorylating various downstream substrates and plays a role in cellular responses. The molecular weight of p38 is approximately 38 kDa. This protein is widely expressed across many tissues including heart brain and lungs. Its activation by stimuli such as cytokines and stress factors helps regulate inflammation and cell cycle control.
P38 is involved in several cellular processes such as inflammation cell differentiation and apoptosis. It often functions as part of a MAPK signaling complex where it serves a critical role in transmitting signals from the cell surface to the nucleus. It interacts with upstream kinases for activation and affects cellular responses by phosphorylating transcription factors and other protein kinases. Through experiments using techniques like p38 western blot and alpha ELISA scientists can monitor its activity and understand its role in cellular physiology.
P38 signaling is integral to both the MAPK and NF-kB pathways. It helps mediate several cellular responses including inflammation and stress responses. Within these pathways p38 interacts with other proteins such as JNK and ERK which helps regulate adaptive and innate immune responses. These interactions ensure distinct yet overlapping signaling responses necessary for cellular homeostasis.
P38 plays a role in conditions such as rheumatoid arthritis and cancer. In rheumatoid arthritis p38 contributes to inflammatory processes promoting the production of pro-inflammatory cytokines. In cancer its role varies; while sometimes promoting cancer cell apoptosis it may also aid in tumor survival and proliferation. The protein TNF-alpha often connects indirectly with p38 through inflammatory pathways highlighting its involvement in these diseases.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com