MEA1 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon 2 and 14 bp insertion in exon 2.
Images
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon 2 and 14 bp insertion in exon 2
Alternative names
HYS, MEA, MEA1_HUMAN, Male enhanced antigen (H Y structural gene), Male-enhanced antigen 1
Recommended products
MEA1 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon 2 and 14 bp insertion in exon 2.
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon 2 and 14 bp insertion in exon 2
- Concentration
Properties
- Gene name
- MEA1
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MEA1 also known as Male-enhanced antigen 1 is a protein that plays a significant role in the process of spermatogenesis. It is a protein composed of approximately 65 kDa. MEA1 is predominantly expressed in the testes indicating its involvement in male reproductive function. The protein has been identified to participate in cellular processes essential to germ cell development and its expression pattern suggests it may have functions that are testis-specific or testis-enhanced.
- Biological function summary
MEA1 interacts with cellular machinery involved in cell cycle regulation and differentiation. Its role has been observed in the development of male germ cells. MEA1 does not form part of a larger protein complex; instead it functions as an independent entity that supports spermatid differentiation. It seems essential for proper morphological changes necessary for mature sperm formation.
- Pathways
Studies show that MEA1 is integral to pathways guiding spermatogenesis and germ cell maturation. It does not directly link to more widespread signaling routes like MAPK or PI3K. MEA1 may interact with proteins such as CREM a transcription factor that regulates genes critical for sperm cell development suggesting it integrates into the transcriptional regulatory framework essential for efficient sperm production.
- Associated diseases and disorders
MEA1’s aberrant expression is associated with male infertility specifically non-obstructive azoospermia which is a condition requiring intervention for sperm production failures. Connections to infertility emphasize the protein's role in maintaining healthy sperm production. Another protein associated with male infertility is DAZL which MEA1 could potentially interact with given their shared involvement in spermatogenesis. Further research could elucidate MEA1’s exact interactions and pathogenic roles connected to reproductive issues.
Product promise
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Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Sanger Sequencing - Human MEA1 knockout HEK-293T cell line (ab266808)
Allele-2: 14 bp insertion in exon 2.
Sanger Sequencing - Human MEA1 knockout HEK-293T cell line (ab266808)
Allele-1: 10 bp deletion in exon 2
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