MEX3B KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon 2 and 1 bp deletion in exon 2 and 1 bp insertion in exon 2.
Images
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon 2 and 1 bp deletion in exon 2 and 1 bp insertion in exon 2
Alternative names
KIAA2009, MEX3B_HUMAN, RING finger and KH domain-containing protein 3, RING finger protein 195, RKHD3, RNA-binding protein MEX3B, RNF195
Recommended products
MEX3B KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon 2 and 1 bp deletion in exon 2 and 1 bp insertion in exon 2.
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Form
- Liquid
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon 2 and 1 bp deletion in exon 2 and 1 bp insertion in exon 2
- Concentration
Properties
- Gene name
- MEX3B
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Handling procedures
- Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.- Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
- Culture medium
- DMEM (High Glucose) + 10% FBS
- Cryopreservation medium
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -196°C
- Appropriate long-term storage conditions
- -196°C
Notes
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MEX3B also known as RNA-binding E3 ubiquitin protein ligase MEX-3B is a protein with a molecular mass of approximately 66 kDa. It functions mechanically as an RNA-binding protein equipped with two K homology (KH) domains and an ubiquitin E3 ligase domain. MEX3B shows expression in various tissues including brain and testis. The protein participates in the post-transcriptional regulation of mRNA influencing stability and localization of its target mRNAs.
- Biological function summary
MEX3B influences cellular processes by regulating gene expression at the post-transcriptional level. It is a component of the ribonucleoprotein complexes involved in mRNA turnover and translation regulation. MEX3B assists in fine-tuning the expression of genes critical for cell differentiation and growth. The protein mediates degradation of selected mRNAs by marking them for decay through the ubiquitin-proteasome pathway.
- Pathways
MEX3B engages in pathways such as Wnt/β-catenin signaling and mTOR signaling. Through these pathways the protein impacts cell proliferation and survival. In the Wnt/β-catenin pathway MEX3B influences AXIN1 degradation affecting β-catenin levels. In mTOR signaling MEX3B collaborates with proteins like PI3K modulating cellular responses to growth factors and nutrients.
- Associated diseases and disorders
Altered expression of MEX3B correlates with cancer and neurological disorders. In cancer MEX3B may potentially influence tumor progression by affecting oncogene and tumor suppressor mRNA stability interacting with proteins such as β-catenin. In neurological disorders its role in mRNA regulation suggests a connection with neurodegenerative conditions often through interactions with mRNA-regulating proteins like HuR.
Product promise
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3 product images
Sanger Sequencing - Human MEX3B knockout HEK-293T cell line (ab267234)
Allele-2: 1 bp insertion in exon 2.
Sanger Sequencing - Human MEX3B knockout HEK-293T cell line (ab267234)
Allele-3: 14 bp deletion in exon 2.
Sanger Sequencing - Human MEX3B knockout HEK-293T cell line (ab267234)
Allele-1: 1 bp deletion in exon2
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