Human MLST8 (G protein beta subunit like/GBL) knockout HCT116 cell line
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- Sanger seq
Unknown
Sanger Sequencing - Human MLST8 (G protein beta subunit like/GBL) knockout HCT116 cell line (AB266868)
Homozygous : 1 bp insertion in exon2
Complementary Products
Cell Lines & Lysates
AB258965
Human MLST8 (G protein beta subunit like/GBL) knockout HCT116 cell lysate
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Product details
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
McCoY5a + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
GBL contributes to the regulation and setting up of signal transduction pathways by forming a part of the G protein complex. This complex involves the alpha beta and gamma subunits where GBL typically interacts with the beta subunit family. By binding to receptors like GPCRs GBL facilitates the exchange of GDP for GTP on the G protein alpha subunit thereby activating downstream effectors. The complex is critical in ensuring precise cellular responses and GBL maintains structural integrity within it.
Pathways
GBL plays an integral role in the G protein-coupled receptor (GPCR) signaling and the MAPK signaling pathways. In GPCR signaling GBL's interaction with the beta subunit influences processes like sensory perception and cell growth. In the MAPK pathway critical for cell responses to growth signals GBL connects with proteins such as RAF and MEK supporting the cascade of phosphorylation events critical for pathway progression. These pathways highlight GBL's importance in cellular dynamics and network interactions.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Target data
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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