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MORF4L2 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 5.

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Sanger Sequencing - Human MORF4L2 (MRGX) knockout HCT116 cell line (AB266861), expandable thumbnail

Key facts

Cell type
HCT116
Species or organism
Human
Tissue
Colon
Form
Liquid
Knockout validation
Sanger Sequencing
Mutation description
Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 5

Alternative names

Recommended products

MORF4L2 KO cell line available to order. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 5.

Key facts

Cell type
HCT116
Form
Liquid
Mutation description
Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 5
Disease
Carcinoma
Concentration
Loading...

Properties

Gene name
MORF4L2
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing
Zygosity
Homozygous

Quality control

STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

Cell culture

Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male

Handling procedures

Initial handling guidelines

Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.

1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.

Subculture guidelines
  • All seeding densities should be based on cell counts gained by established methods.
  • A guide seeding density of 2x104 cells/cm2 is recommended.
  • Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
McCoY5a + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.

Storage

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-196°C
Appropriate long-term storage conditions
-196°C

Notes

Recommended control: Human wild-type HCT116 cell line (ab255451). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.

We will provide viable cells that proliferate on revival.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

MRGX also known as Mas-related G protein-coupled receptor member X functions as a G protein-coupled receptor (GPCR) involved in sensing environmental stimuli and transducing signals inside cells. Researchers find this receptor in sensory neurons within the dorsal root ganglia and trigeminal ganglia. MRGX weighs approximately 37 kDa and plays a role in pain perception and modulation by interacting with various ligands. Understanding its mechanical function helps in exploring its wide applications in sensory biology.

Biological function summary

This receptor contributes to the modulation of nociceptive signals and influences pain sensation and response. MRGX does not form part of a complex; it functions independently to modulate pain pathways by interacting with peptides and other small molecules. It also regulates calcium ion concentrations within neurons which is essential for neurotransmission and signal propagation. The interaction of MRGX with these elements highlights its significant contribution to the sensory regulatory processes.

Pathways

Research associates MRGX with pain perception and sensory transmission pathways. It intersects with the neuropeptide signaling pathway influencing proteins such as substance P and calcitonin gene-related peptide (CGRP) both key players in pain signal pathways. MRGX modulates the activity of these signaling molecules affecting how the body interprets and reacts to painful stimuli. This receptor's involvement in these pathways demonstrates its impact on the physiological responses to pain.

Associated diseases and disorders

MRGX is linked to conditions such as neuropathic pain and inflammatory pain. Alterations in MRGX function or expression can influence the severity of pain-related phenomena implicating it in these conditions. The association of MRGX with proteins like TRPV1 and Nav1.8 which are involved in pain pathways further solidifies its connection to these disorders. Understanding these interactions offers insights into potential therapeutic avenues for treating chronic pain syndromes.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Sanger Sequencing - Human MORF4L2 (MRGX) knockout HCT116 cell line (ab266861), expandable thumbnail

    Sanger Sequencing - Human MORF4L2 (MRGX) knockout HCT116 cell line (ab266861)

    Homozygous: 1 bp deletion in exon5

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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