Human MPP1 knockout HEK-293T cell line
- Advanced Validation
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MPP1 KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 13 bp deletion in exon 6. To order both knockout and wild-type control cells: select '2 x 1000000 Cells/vial'. To order only knockout cells: select '1000000 Cells/vial'.
View Alternative Names
55 kDa erythrocyte membrane protein, AAG 12, Aging associated gene 12, DXS552, DXS552E, EM55_HUMAN, EMP 55, Erythrocyte membrane protein p55, MRG-1, Membrane protein, Membrane protein palmitoylated 1, Membrane protein palmitoylated 1 55kDa, Migration related gene 1, OTTHUMP00000026054, OTTHUMP00000196176, PEMP, Palmitoylated erythrocyte membrane protein, Palmitoylated membrane protein 1, p55, palmitoylated 1
- WB
Lab
Western blot - Human MPP1 knockout HEK-293T cell line (AB267248)
Lanes 1-4 : Merged signal (red and green). Green - ab108528 observed at 52 kDa. Red - loading control ab8245 observed at 36 kDa.
ab108528 Anti-MPP1 antibody [EPR5865] was shown to specifically react with MPP1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab267248 (knockout cell lysate ab258051) was used. Wild-type and MPP1 knockout samples were subjected to SDS-PAGE. ab108528 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4° at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-MPP1 antibody [EPR5865] (<a href='/en-us/products/primary-antibodies/mpp1-antibody-epr5865-ab108528'>ab108528</a>) at 1/1000 dilution
Lane 1:
Wild-type HEK293T cell lysate at 20 µg
Lane 2:
MPP1 knockout HEK293T cell lysate at 20 µg
Lane 2:
Western blot - Human MPP1 knockout HEK-293T cell line (ab267248)
Lane 3:
Jurkat cell lysate at 20 µg
Lane 4:
K-562 cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa
false
- WB
Lab
Western blot - Human MPP1 knockout HEK-293T cell line (AB267248)
Lanes 1-4 : Merged signal (red and green). Green - ab133500 observed at 52 kDa. Red - loading control ab8245 observed at 36 kDa.
ab133500 Anti-MPP1 antibody [EPR5864] was shown to specifically react with MPP1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab267248 (knockout cell lysate ab258051) was used. Wild-type and MPP1 knockout samples were subjected to SDS-PAGE. ab133500 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4° at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-MPP1 antibody [EPR5864] (<a href='/en-us/products/primary-antibodies/mpp1-antibody-epr5864-ab133500'>ab133500</a>) at 1/1000 dilution
Lane 1:
Wild-type HEK293T cell lysate at 20 µg
Lane 2:
MPP1 knockout HEK293T cell lysate at 20 µg
Lane 2:
Western blot - Human MPP1 knockout HEK-293T cell line (ab267248)
Lane 3:
Jurkat cell lysate at 20 µg
Lane 4:
K-562 cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa
false
- Cell Culture
Unknown
Cell Culture - Human MPP1 knockout HEK-293T cell line (AB267248)
Representative images of MPP1 knockout HEK293T cells, low and high confluency examples (top left and right respectively) and wild-type HEK293T cells, low and high confluency (bottom left and right respectively) showing typical adherent, epithelial-like morphology. Images were captured at 10X magnification using an EVOS M5000 microscope.
- Sanger seq
Unknown
Sanger Sequencing - Human MPP1 knockout HEK-293T cell line (AB267248)
Homozygous : 13 bp deletion in exon6
Reactivity data
Product details
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Handling procedures
Initial handling guidelines
Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.
1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.
Subculture guidelines
- All seeding densities should be based on cell counts gained by established methods.
- A guide seeding density of 2x104 cells/cm2 is recommended.
- Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
In various cell types MPP1 supports cell adhesion and communication. As a complex-forming protein MPP1 interacts with spectrin and actin contributing to the structural organization of cell membranes. MPP1 regulates interactions within the cellular environment influencing cellular signaling and mechanical integrity. By establishing these connections it helps maintain cell shape and facilitate cell signaling processes.
Pathways
Several interactions actively involve MPP1. Within the pathways regulating cell shape MPP1 contributes to the RhoA signaling pathway important for cytoskeletal organization. Furthermore MPP1 closely works with proteins like Ezrin to support plasma membrane stability and intracellular communication. These interactions are significant for maintaining normal cellular junctions and morphology.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Target data
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com