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MPP1 KO cell line available to order. KO validated by Western blot. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 13 bp deletion in exon 6.

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Images

Western blot - Human MPP1 knockout HEK-293T cell line (AB267248), expandable thumbnail
  • Western blot - Human MPP1 knockout HEK-293T cell line (AB267248), expandable thumbnail
  • Cell Culture - Human MPP1 knockout HEK-293T cell line (AB267248), expandable thumbnail
  • Sanger Sequencing - Human MPP1 knockout HEK-293T cell line (AB267248), expandable thumbnail

Key facts

Cell type
HEK-293T
Species or organism
Human
Tissue
Kidney
Form
Liquid
Knockout validation
Sanger Sequencing, Western blot
Mutation description
Knockout achieved by using CRISPR/Cas9, Homozygous: 13 bp deletion in exon 6

Alternative names

55 kDa erythrocyte membrane protein, AAG 12, Aging associated gene 12, DXS552, DXS552E, EM55_HUMAN, EMP 55, Erythrocyte membrane protein p55, MRG-1, Membrane protein, Membrane protein palmitoylated 1, Membrane protein palmitoylated 1 55kDa, Migration related gene 1, OTTHUMP00000026054, OTTHUMP00000196176, PEMP, Palmitoylated erythrocyte membrane protein, Palmitoylated membrane protein 1, p55, palmitoylated 1

Recommended products

MPP1 KO cell line available to order. KO validated by Western blot. Free of charge wild type control provided. Knockout achieved by using CRISPR/Cas9, Homozygous: 13 bp deletion in exon 6.

Key facts

Cell type
HEK-293T
Form
Liquid
Mutation description
Knockout achieved by using CRISPR/Cas9, Homozygous: 13 bp deletion in exon 6
Concentration
Loading...

Properties

Gene name
MPP1
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Zygosity
Homozygous

Quality control

STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

Cell culture

Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female

Handling procedures

Initial handling guidelines

Upon arrival, the vial should be stored in liquid nitrogen vapor phase and not at -80°C. Storage at -80°C may result in loss of viability.

1. Thaw the vial in 37°C water bath for approximately 1-2 minutes.
2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. 10 mL represents minimum recommended dilution. 20 mL represents maximum recommended dilution.
3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method seed all remaining cells into a T25.
4. Incubate the culture at 37°C incubator with 5% CO2. Check the culture one day after revival and continue to check until 80% confluent. Media change can be given if needed.
5. Once confluent passage into an appropriate flask at a density of 2x104 cells/cm2. Seeding density is given as a guide only and should be scaled to align with individual lab schedules. Cultures should be monitored daily.

Subculture guidelines
  • All seeding densities should be based on cell counts gained by established methods.
  • A guide seeding density of 2x104 cells/cm2 is recommended.
  • Cells should be passaged when they have achieved 80-90% confluence.
Culture medium
DMEM (High Glucose) + 10% FBS
Cryopreservation medium
Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose.

Storage

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-196°C
Appropriate long-term storage conditions
-196°C

Notes

Recommended control: Human wild-type HEK293T cell line (ab255449). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.

We will provide viable cells that proliferate on revival.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The MPP1 protein also known as Membrane Palmitoylated Protein 1 acts as a part of the membrane-associated guanylate kinase (MAGUK) family. This protein has a molecular mass of around 55 kDa. MPP1 expresses mainly in erythrocytes the cells that carry oxygen in the blood. Functionally it plays a role in maintaining cell shape and stability by contributing to the cytoskeletal structure. It localizes at the plasma membrane and interacts with other proteins to fulfill its functions.

Biological function summary

In various cell types MPP1 supports cell adhesion and communication. As a complex-forming protein MPP1 interacts with spectrin and actin contributing to the structural organization of cell membranes. MPP1 regulates interactions within the cellular environment influencing cellular signaling and mechanical integrity. By establishing these connections it helps maintain cell shape and facilitate cell signaling processes.

Pathways

Several interactions actively involve MPP1. Within the pathways regulating cell shape MPP1 contributes to the RhoA signaling pathway important for cytoskeletal organization. Furthermore MPP1 closely works with proteins like Ezrin to support plasma membrane stability and intracellular communication. These interactions are significant for maintaining normal cellular junctions and morphology.

Associated diseases and disorders

MPP1 links to specific conditions with impacts on erythrocyte function. It has been implicated in hereditary spherocytosis a disorder affecting red blood cell stability and causing anemia. MPP1 also interacts with the protein Ankyrin an important component in maintaining erythrocyte membrane structure. These associations highlight the importance of MPP1 in blood-related diseases marking it as an important target for understanding membrane disorders.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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